Structure of PDB 7p5x Chain AD

Receptor sequence

>7p5xAD (length=1265) Species: 246196 (Mycolicibacterium smegmatis MC2 155)

MLDVNFFDELRIGLATADDIRNWSYGEVKKPETINYRTLKPEKDGLFCEK
IFGPTRDWECYCGKYKRVRFKGIICERCGVEVTRAKVRRERMGHIELAAP
VTHIWYFKGVPSRLGYLLDLAPKDLEKIIYFAAYVITSVDDEMRHNELST
LEAEMAVEKKAVEDQRDADLEARAQKLEADLAELEAEGAKSDVRRKVRDS
GEREMRQLRDRAQRELDRLDEIWNTFTKLAPKQLIVDEVLYRELQDRYGE
YFTGAMGAESIKKLIENFDIDAEAESLREVIRSGKGQKKLRALKRLKVVA
AFQQSGNSPMGMVLDAVPVIPPELRPMVQLDGGRFATSDLNDLYRRVINR
NNRLKRLIDLGAPEIIVNNEKRMLQESVDALFDNGRRGRPVTGPGNRPLK
SLSDLLKGKQGRFRQNLLGKRVDYSGRSVIVVGPQLKLHQCGLPKLMALE
LFKPFVMKRLVDLNHAQNIKSAKRMVERQRPQVWDVLEEVIAEHPVLLNR
APTLHRLGIQAFEPQLVEGKAIQLHPLVCEAFNADFDGDQMAVHLPLSAE
AQAEARILMLSSNNILSPASGKPLAMPRLDMVTGLYYLTTLVEGATGEYQ
AATKDAPEQGVYSSPAEAIMAMDRGALSVRAKIKVRLTELRPPTDLEAQL
FENGWKPGDAWTAETTLGRVMFNELLPKSYPFVNEQMHKKVQARIINDLA
ERFPMIVVAQTVDKLKDAGFYWATRSGVTVSMADVLVPPQKQEILERHEA
EADAIERKYQRGALNHTERNESLVKIWQDATEEVGKALEEFYPADNPIIT
IVKSGATGNLTQTRTLAGMKGLVTNPKGEFIPRPIKSSFREGLTVLEYFI
NTHGARKGLADTALRTADSGYLTRRLVDVSQDVIVREHDCETERGINVTL
AERGPDGTLIRDAHVETSAFARTLATDAVDANGNVIIERGHDLGDPAIDA
LLAAGITTVKVRSVLTCTSATGVCAMCYGRSMATGKLVDIGEAVGIVAAQ
SIGEPGTQLTMRVGGLPRVQELFEARVPRNKAPIADVAGRVRLEESDKFF
KITIVPDDGGEEVVYDKLSKRQRLRVITHGVLSDGDHVEVGDQLMEGAAD
PHEVLRVQGPREVQIHLVKEVQEVYRAQGVSIHDKHIEVIVRQMLRRVTI
IDSGSTEFLPGSLTERAEFEAENRRVVAEGGEPAAGRPVLMGITKASLAT
DSWLSAASFQETTRVLTDAAINCRSDKLNGLKENVIIGKLIPAGTGISRY
RNIQVQPTEEARAAA

3D structure

• PDB: 7p5x Transcriptional control of mycobacterial DNA damage response by sigma adaptation.
• Chain: AD
• Resolution: 3.2 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.6: DNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	AD	Y36 K294 R1039	Y36 K294 R1026
BS02	dna	AD	L330 G395 K409 R421 T866 A867 Y871 Q1228 E1229	L330 G395 K409 R421 T866 A867 Y871 Q1210 E1211
BS03	ZN	AD	C890 C967 C977	C890 C967 C977
BS04	ZN	AD	C60 C75 C78	C60 C75 C78

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003677 DNA binding
• GO:0003899 DNA-directed 5'-3' RNA polymerase activity
• GO:0008270 zinc ion binding
• GO:0016779 nucleotidyltransferase activity
• GO:0034062 5'-3' RNA polymerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006351 DNA-templated transcription

Cellular Component

• GO:0000428 DNA-directed RNA polymerase complex

External links

• PDB: RCSB:7p5x, PDBe:7p5x, PDBj:7p5x
• PDBsum: 7p5x
• PubMed: 34851662
• UniProt: A0QS66|RPOC_MYCS2 DNA-directed RNA polymerase subunit beta' (Gene Name=rpoC)