Structure of PDB 8g8p Chain AAA

Receptor sequence

>8g8pAAA (length=248) Species: 224325 (Archaeoglobus fulgidus DSM 4304)

RVEVFPVEGLPLIKEGDDLAELISSRVRFEDGDVLVVCSTVISKAEGRIR
RLEEFNPSERAKEIAARIGKPAEFVQAVLEESEEVLLDFPFLLVKAKFGN
VCVNAGIDASNVEEGSLLLPPLDPDGSAEKLRRRILELTGKRVGVIITDT
NGRCFRRGVVGFAIGISGVKAMKDWIGRKDLYGRELEVTVECVADEIAAF
ANLLMGEGGDGIPAVVVRGLNVAGEGSMEEIYRSEEEDVIRRCLKRCL

3D structure

• PDB: 8g8p Poly-gamma-glutamylation of biomolecules.
• Chain: AAA
• Resolution: 1.83 Å

Enzymatic activity

• Enzyme Commision number: 6.3.2.31: coenzyme F420-0:L-glutamate ligase.
  6.3.2.34: coenzyme F420-1:gamma-L-glutamate ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	F42	AAA	F92 V104 N105 D109 A110 S111 T151 G153 R154 C155	F91 V103 N104 D108 A109 S110 T150 G152 R153 C154
BS02	GTP	AAA	L11 P12 I14 C39 S40 T41 V42 K45 D109 N112 D150 T151 M206 E208 G209 G212 I213 P214	L10 P11 I13 C38 S39 T40 V41 K44 D108 N111 D149 T150 M205 E207 G208 G211 I212 P213
BS03	GDP	AAA	L11 P12 L13 I14 C39 S40 T41 V42 K45 N112 D150 M206 G209 G212 I213 P214	L10 P11 L12 I13 C38 S39 T40 V41 K44 N111 D149 M205 G208 G211 I212 P213
BS04	MN	AAA	T151 E208	T150 E207
BS05	MN	AAA	D109 D150	D108 D149
BS06	MN	AAA	D109 D150	D108 D149

Gene Ontology

Molecular Function

• GO:0005525 GTP binding
• GO:0016874 ligase activity
• GO:0043773 coenzyme F420-0 gamma-glutamyl ligase activity
• GO:0046872 metal ion binding
• GO:0052618 coenzyme F420-0:L-glutamate ligase activity
• GO:0052619 coenzyme F420-1:gamma-L-glutamate ligase activity

Biological Process

• GO:0052645 F420-0 metabolic process

External links

• PDB: RCSB:8g8p, PDBe:8g8p, PDBj:8g8p
• PDBsum: 8g8p
• PubMed: 38346985
• UniProt: O28028|COFE_ARCFU Coenzyme F420:L-glutamate ligase (Gene Name=cofE)