Structure of PDB 8ajs Chain AAA

Receptor sequence

>8ajsAAA (length=461) Species: 208964 (Pseudomonas aeruginosa PAO1)

GFTDYKVADITLAAWGRRELIIAESEMPALMGLRRKYAGQQPLKGAKILG
CIHMTIQTGVLIETLVALGAEVRWSSCNIFSTQDQAAAAIAAAGIPVFAW
KGETEEEYEWCIEQTILKDGQPWDANMVLDDGGDLTEILHKKYPQMLERI
HGITEETTTGVHRLLDMLKNGTLKVPAINVNDSVTKSKNDNKYGCRHSLN
DAIKRGTDHLLSGKQALVIGYGDVGKGSSQSLRQEGMIVKVAEVDPICAM
QACMDGFEVVSPYKNGINDGTEASIDAALLGKIDLIVTTTGNVNVCDANM
LKALKKRAVVCNIGHADNEIDTAFMRKNWAWEEVKPQVHKIHRTGKDGFD
AHNDDYLILLAEGRLVNLGNATGHPSRIMDGSFANQVLAQIHLFEQKYAD
LPAAEKAKRLSVEVLPKKLDEEVALEMVKGFGGVVTQLTPKQAEYIGVSV
EGPFKPDTYRY

3D structure

• PDB: 8ajs Crystal structure of the F324A mutant of S-adenosyl-L-homocysteine hydrolase from Pseudomonas aeruginosa cocrystallized with adenosine in the presence of K+ cations
• Chain: AAA
• Resolution: 1.68 Å

Enzymatic activity

• Enzyme Commision number: 3.13.2.1: adenosylhomocysteinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NAD	AAA	T166 T167 N199 I227 G230 D231 V232 E251 V252 T298 N300 I321 G322 N375 H382	T158 T159 N191 I219 G222 D223 V224 E243 V244 T290 N292 I313 G314 N367 H374
BS02	ADN	AAA	H61 T63 Q65 D139 E164 T165 K194 D198 L373 L376 H382 M387	H53 T55 Q57 D131 E156 T157 K186 D190 L365 L368 H374 M379
BS03	NAD	AAA	K463 Y467	K455 Y459

Gene Ontology

Molecular Function

• GO:0004013 adenosylhomocysteinase activity
• GO:0016787 hydrolase activity

Biological Process

• GO:0006730 one-carbon metabolic process
• GO:0033353 S-adenosylmethionine cycle
• GO:0071269 L-homocysteine biosynthetic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:8ajs, PDBe:8ajs, PDBj:8ajs
• PDBsum: 8ajs
• UniProt: Q9I685|SAHH_PSEAE Adenosylhomocysteinase (Gene Name=ahcY)