Structure of PDB 7z3l Chain AAA

Receptor sequence

>7z3lAAA (length=787) Species: 10116 (Rattus norvegicus)

GSCKGRCFELQEVGPPDCRCDNLCKSYSSCCHDFDELCLKTARGWECTKD
RCGEVRNEENACHCSEDCLSRGDCCTNYQVVCKGESHWVDDDCEEIKVPE
CPAGFVRPPLIIFSVDGFRASYMKKGSKVMPNIEKLRSCGTHAPYMRPVY
PTKTFPNLYTLATGLYPESHGIVGNSMYDPVFDASFHLRGREKFNHRWWG
GQPLWITATKQGVRAGTFFWSVSIPHERRILTILQWLSLPDNERPSVYAF
YSEQPDFSGHKYGPFGPEMTNPLREIDKTVGQLMDGLKQLRLHRCVNVIF
VGDHGMEDVTCDRTEFLSNYLTNVDDITLVPGTLGRIRAKSINNSKYDPK
TIIAALTCKKPDQHFKPYMKQHLPKRLHYANNRRIEDIHLLVDRRWHVAR
KPLDVSGKCFFQGDHGFDNKVNSMQTVFVGYGPTFKYRTKVPPFENIELY
NVMCDLLGLKPAPNNGTHGSLNHLLRTNTFRPTMPDEVSRPNYPGIMYLQ
SEFDLGCTCDDRLHTKGSTKERHLLYGRPAVLYRTSYDILYHTDFESGYS
EIFLMPLWTSYTISKQAEVSSIPEHLTNCVRPDVRVSPGFSQNCLAYKND
KQMSYGFLFPPYLSSSPEAKYDAFLVTNMVPMYPAFKRVWAYFQRVLVKK
YASERNGVNVISGPIFDYNYDGLRDTEDEIKQYVEGSSIPVPTHYYSIIT
SCLDFTQPADKCDGPLSVSSFILPHRPDNDESCASSEDESKWVEELMKMH
TARVRDIEHLTGLDFYRKTSRSYSEILTLKTYLHTYE

3D structure

• PDB: 7z3l Autotaxin facilitates selective LPA receptor signaling.
• Chain: AAA
• Resolution: 2.4 Å

Enzymatic activity

• Enzyme Commision number: 3.1.4.39: alkylglycerophosphoethanolamine phosphodiesterase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MAN	AAA	S224 G255	S169 G200
BS02	MAN	AAA	E223 N436	E168 N381
BS03	MAN	AAA	Q257 K265	Q202 K210
BS04	ZN	AAA	D311 H315 H474	D256 H260 H415
BS05	ZN	AAA	D171 T209 D358 H359	D116 T154 D303 H304
BS06	CA	AAA	D739 N741 D743 L745 D747	D667 N669 D671 L673 D675
BS07	7NB	AAA	I167 F210 L213 K248 F249 H251 W254 W260 F273 F274	I112 F155 L158 K193 F194 H196 W199 W205 F218 F219

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0004528 phosphodiesterase I activity
• GO:0004622 lysophospholipase activity
• GO:0004630 phospholipase D activity
• GO:0005509 calcium ion binding
• GO:0008270 zinc ion binding
• GO:0016787 hydrolase activity
• GO:0016788 hydrolase activity, acting on ester bonds
• GO:0046872 metal ion binding
• GO:0047391 alkylglycerophosphoethanolamine phosphodiesterase activity

Biological Process

• GO:0001953 negative regulation of cell-matrix adhesion
• GO:0006644 phospholipid metabolic process
• GO:0006935 chemotaxis
• GO:0008284 positive regulation of cell population proliferation
• GO:0009395 phospholipid catabolic process
• GO:0010634 positive regulation of epithelial cell migration
• GO:0016042 lipid catabolic process
• GO:0016192 vesicle-mediated transport
• GO:0030149 sphingolipid catabolic process
• GO:0030334 regulation of cell migration
• GO:0034638 phosphatidylcholine catabolic process
• GO:0044849 estrous cycle
• GO:0048714 positive regulation of oligodendrocyte differentiation
• GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
• GO:0051894 positive regulation of focal adhesion assembly
• GO:0060326 cell chemotaxis
• GO:0071276 cellular response to cadmium ion
• GO:0071392 cellular response to estradiol stimulus
• GO:1900026 positive regulation of substrate adhesion-dependent cell spreading
• GO:1903165 response to polycyclic arene
• GO:2000394 positive regulation of lamellipodium morphogenesis

Cellular Component

• GO:0005576 extracellular region
• GO:0005615 extracellular space

External links

• PDB: RCSB:7z3l, PDBe:7z3l, PDBj:7z3l
• PDBsum: 7z3l
• PubMed: 36640760
• UniProt: Q64610|ENPP2_RAT Autotaxin (Gene Name=Enpp2)