Structure of PDB 7z3k Chain AAA

Receptor sequence

>7z3kAAA (length=791) Species: 10116 (Rattus norvegicus)

GSCKGRCFELQEVGPPDCRCDNLCKSYSSCCHDFDELCLKTARGWECTKD
RCGEVRNEENACHCSEDCLSRGDCCTNYQVVCKGESHWVDDDCEEIKVPE
CPAGFVRPPLIIFSVDGFRASYMKKGSKVMPNIEKLRSCGTHAPYMRPVY
PTKTFPNLYTLATGLYPESHGIVGNSMYDPVFDASFHLRGREKFNHRWWG
GQPLWITATKQGVRAGTFFWSVSIPHERRILTILQWLSLPDNERPSVYAF
YSEQPDFSGHKYGPFGPEMTNPLREIDKTVGQLMDGLKQLRLHRCVNVIF
VGDHGMEDVTCDRTEFLSNYLTNVDDITLVPGTLGRIRAKSINNSKYDPK
TIIAALTCKKPDQHFKPYMKQHLPKRLHYANNRRIEDIHLLVDRRWHVAR
KPLDKCFFQGDHGFDNKVNSMQTVFVGYGPTFKYRTKVPPFENIELYNVM
CDLLGLKPAPNNGTHGSLNHLLRTNTFRPTMPDEVSRPNYPGIMYLQSEF
DLGCTCDDKLEELNKRLHTKGSTKERHLLYGRPAVLYRTSYDILYHTDFE
SGYSEIFLMPLWTSYTISKQAEVSSIPEHLTNCVRPDVRVSPGFSQNCLA
YKNDKQMSYGFLFPPYLSSSPEAKYDAFLVTNMVPMYPAFKRVWAYFQRV
LVKKYASERNGVNVISGPIFDYNYDGLRDTEDEIKQYVEGSSIPVPTHYY
SIITSCLDFTQPADKCDGPLSVSSFILPHRPDNDESCASSEDESKWVEEL
MKMHTARVRDIEHLTGLDFYRKTSRSYSEILTLKTYLHTYE

3D structure

• PDB: 7z3k Autotaxin facilitates selective LPA receptor signaling.
• Chain: AAA
• Resolution: 2.0 Å

Enzymatic activity

• Enzyme Commision number: 3.1.4.39: alkylglycerophosphoethanolamine phosphodiesterase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	AAA	D311 H315 H474	D256 H260 H412
BS02	ZN	AAA	D171 D311 H359 H474	D116 D256 H304 H412
BS03	5JK	AAA	Y214 K248 F249 H251 W254 P258 I261 F274	Y159 K193 F194 H196 W199 P203 I206 F219
BS04	CA	AAA	D739 N741 D743 L745 D747	D671 N673 D675 L677 D679
BS05	IA0	AAA	T209 F210 L213 N230 L243 F273 F274 W275 Y306 E308 D311 H474	T154 F155 L158 N175 L188 F218 F219 W220 Y251 E253 D256 H412

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0004528 phosphodiesterase I activity
• GO:0004622 lysophospholipase activity
• GO:0004630 phospholipase D activity
• GO:0005509 calcium ion binding
• GO:0008270 zinc ion binding
• GO:0016787 hydrolase activity
• GO:0016788 hydrolase activity, acting on ester bonds
• GO:0046872 metal ion binding
• GO:0047391 alkylglycerophosphoethanolamine phosphodiesterase activity

Biological Process

• GO:0001953 negative regulation of cell-matrix adhesion
• GO:0006644 phospholipid metabolic process
• GO:0006935 chemotaxis
• GO:0008284 positive regulation of cell population proliferation
• GO:0009395 phospholipid catabolic process
• GO:0010634 positive regulation of epithelial cell migration
• GO:0016042 lipid catabolic process
• GO:0016192 vesicle-mediated transport
• GO:0030149 sphingolipid catabolic process
• GO:0030334 regulation of cell migration
• GO:0034638 phosphatidylcholine catabolic process
• GO:0044849 estrous cycle
• GO:0048714 positive regulation of oligodendrocyte differentiation
• GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
• GO:0051894 positive regulation of focal adhesion assembly
• GO:0060326 cell chemotaxis
• GO:0071276 cellular response to cadmium ion
• GO:0071392 cellular response to estradiol stimulus
• GO:1900026 positive regulation of substrate adhesion-dependent cell spreading
• GO:1903165 response to polycyclic arene
• GO:2000394 positive regulation of lamellipodium morphogenesis

Cellular Component

• GO:0005576 extracellular region
• GO:0005615 extracellular space

External links

• PDB: RCSB:7z3k, PDBe:7z3k, PDBj:7z3k
• PDBsum: 7z3k
• PubMed: 36640760
• UniProt: Q64610|ENPP2_RAT Autotaxin (Gene Name=Enpp2)