Structure of PDB 6zic Chain AAA

Receptor sequence

>6zicAAA (length=723) Species: 10116 (Rattus norvegicus)

PRGSHMAEYLRLPHSLAMIRLCNPPVNAVSPTVIREVRNGLQKAGSDHTV
KAIVICGANGNFCAGADIHGFSAFTPGLALGSLVDEIQRYQKPVLAAIQG
VALGGGLELALGCHYRIANAKARVGLPEVTLGILPGARGTQLLPRVVGVP
VALDLITSGKYLSADEALRLGILDAVVKSDPVEEAIKFAQKIIDKPIEPR
RIFNKPVPSLPNMDSVFAEAIAKVRKQYPGVLAPETCVRSIQASVKHPYE
VGIKEEEKLFMYLRASGQAKALQYAFFAEKSANKWSTPSGASWKTASAQP
VSSVGVLGLGTMGRGIAISFARVGISVVAVESDPKQLDAAKKIITFTLEK
EASRAHQNGSAKPKLRFSSSTKELSTVDLVVEAVFEDMNLKKKVFAELSA
LCKPGAFLCTNTSALNVDDIASSTDRPQLVIGTHFFSPAHVMRLLEVIPS
RYSSPTTIATVMSLSKKIGKIGVVVGNCYGFVGNRMLAPYYNQGFFLLEE
GSKPEDVDGVLEEFGFKMGPFRVSDLAGLDVGWKIRKGQGLTGPSLPPGT
PVRKRGNSRYSPLGDMLCEAGRFGQKTGKGWYQYDKPLGRIHKPDPWLST
FLSQYREVHHIEQRTISKEEILERCLYSLINEAFRILEEGMAARPEHIDV
IYLHGYGWPRHKGGPMFYAASVGLPTVLEKLQKYYRQNPDIPQLEPSDYL
RRLVAQGSPPLKEWQSLAGPHGS

3D structure

• PDB: 6zic CRYSTAL STRUCTURE OF RAT PEROXISOMAL MULTIFUNCTIONAL ENZYME TYPE-1 (RPMFE1) COMPLEXED WITH 3S-HYDROXYBUTANOYL-COA AND NADH
• Chain: AAA
• Resolution: 2.2 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): A61 F66 G76 G100 E103 P122 E123 G131 K249 S410 H431 E443 N481
• Catalytic site (residue number reindexed from 1): A66 F71 G81 G105 E108 P127 E128 G136 K254 S413 H434 E446 N484
• Enzyme Commision number: 1.1.1.35: 3-hydroxyacyl-CoA dehydrogenase.
  4.2.1.17: enoyl-CoA hydratase.
  5.3.3.8: Delta(3)-Delta(2)-enoyl-CoA isomerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	3HC	AAA	P20 V21 A23 A61 D62 I63 G99 G100 E103 E123 Y156	P25 V26 A28 A66 D67 I68 G104 G105 E108 E128 Y161
BS02	NAI	AAA	G303 G305 T306 M307 E326 S327 Q331 A380 V381 F382 E383 L387 K388 N408 S410 H431	G308 G310 T311 M312 E331 S332 Q336 A383 V384 F385 E386 L390 K391 N411 S413 H434

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0003857 3-hydroxyacyl-CoA dehydrogenase activity
• GO:0004165 delta(3)-delta(2)-enoyl-CoA isomerase activity
• GO:0004300 enoyl-CoA hydratase activity
• GO:0016491 oxidoreductase activity
• GO:0016509 long-chain-3-hydroxyacyl-CoA dehydrogenase activity
• GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
• GO:0016829 lyase activity
• GO:0016853 isomerase activity
• GO:0016863 intramolecular oxidoreductase activity, transposing C=C bonds
• GO:0019899 enzyme binding
• GO:0070403 NAD+ binding

Biological Process

• GO:0006631 fatty acid metabolic process
• GO:0006635 fatty acid beta-oxidation

Cellular Component

• GO:0005777 peroxisome
• GO:0005829 cytosol

External links

• PDB: RCSB:6zic, PDBe:6zic, PDBj:6zic
• PDBsum: 6zic
• UniProt: P07896|ECHP_RAT Peroxisomal bifunctional enzyme (Gene Name=Ehhadh)