Structure of PDB 6zfa Chain AAA

Receptor sequence

>6zfaAAA (length=358) Species: 9606 (Homo sapiens)

LNNYLHVFYYSWYGNPQFDGKYIHWNHPVLEHWDPRIAKNYPQGRHNPPD
DIGSSFYPELGSYSSRDPSVIETHMRQMRSASIGVLALSWYPPDVNDENG
EPTDNLVPTILDKAHKYNLKVTFHIEPYSNRDDQNMYKNVKYIIDKYGNH
PAFYRYKTKTGNALPMFYVYDSYITKPEKWANLLTTSGSRSIRNSPYDGL
FIALLVEEKHKYDILQSGFDGIYTYFATNGFTYGSSHQNWASLKLFCDKY
NLIFIPSVGPGYIDTSIRPWNTQNTRNRINGKYYEIGLSAALQTRPSLIS
ITSFNEWHEGTQIEKAVPKRTSNTVYLDYRPHKPGLYLELTRKWSEKYSK
ERATYALD

3D structure

• PDB: 6zfa Structure of human endo-alpha-1,2-mannosidase (MANEA), an antiviral host-glycosylation target.
• Chain: AAA
• Resolution: 1.8 Å

Enzymatic activity

• Enzyme Commision number: 3.2.1.130: glycoprotein endo-alpha-1,2-mannosidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MAN	AAA	Y268 D269 Y271	Y170 D171 Y173
BS02	MAN	AAA	F329 Y360 R366 W368 N369 E407	F231 Y262 R268 W270 N271 E309
BS03	TEW	AAA	Y254 T256 K257 T258 A454 L455 D456	Y156 T158 K159 T160 A356 L357 D358
BS04	TEW	AAA	K309 F344 K347	K211 F246 K249
BS05	IFM	AAA	Y108 H222 E224 Y268 Y323 E407	Y10 H124 E126 Y170 Y225 E309
BS06	GLC	AAA	H125 D195 E196 N197 I365 R366 E404 H406 E407	H27 D97 E98 N99 I267 R268 E306 H308 E309

Gene Ontology

Molecular Function

• GO:0016798 hydrolase activity, acting on glycosyl bonds

External links

• PDB: RCSB:6zfa, PDBe:6zfa, PDBj:6zfa
• PDBsum: 6zfa
• PubMed: 33154157
• UniProt: Q5SRI9|MANEA_HUMAN Glycoprotein endo-alpha-1,2-mannosidase (Gene Name=MANEA)