Structure of PDB 6wks Chain AAA

Receptor sequence

>6wksAAA (length=298) Species: 2697049 (Severe acute respiratory syndrome coronavirus 2)

SSQAWQPGVAMPNLYKMQRMLLEKCDLQNYGDSATLPKGIMMNVAKYTQL
CQYLNTLTLAVPYNMRVIHFGAGSDKGVAPGTAVLRQWLPTGTLLVDSDL
NDFVSDADSTLIGDCATVHTANKWDLIISDMYDPKTKNVTKENDSKEGFF
TYICGFIQQKLALGGSVAIKITEHSWNADLYKLMGHFAWWTAFVTNVNAS
SSEAFLIGCNYLGKPREQIDGYVMHANYIFWRNTNPIQLSSYSLFDMSKF
PLKLRGTAVMSLKEGQINDMILSLLSKGRLIIRENNRVVISSDVLVNN

3D structure

• PDB: 6wks Structural basis of RNA cap modification by SARS-CoV-2.
• Chain: AAA
• Resolution: 1.8 Å

Enzymatic activity

• Enzyme Commision number: 2.1.1.56: mRNA (guanine-N(7))-methyltransferase.
  2.1.1.57: methyltransferase cap1.
  2.7.7.48: RNA-directed RNA polymerase.
  2.7.7.50: mRNA guanylyltransferase.
  3.1.13.-
  3.4.19.12: ubiquitinyl hydrolase 1.
  3.4.22.-
  3.4.22.69: SARS coronavirus main proteinase.
  3.6.4.12: DNA helicase.
  3.6.4.13: RNA helicase.
  4.6.1.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SAM	AAA	N43 G71 G73 P80 G81 D99 L100 D114 C115 D130 M131 Y132	N43 G71 G73 P80 G81 D99 L100 D114 C115 D130 M131 Y132
BS02	ADN	AAA	L57 T58 W189	L57 T58 W189
BS03	GTA	AAA	C25 D26 L27 Y30 K46 Y132 K137 K170 T172 E173 H174 S201 S202	C25 D26 L27 Y30 K46 Y132 K137 K170 T172 E173 H174 S201 S202

Gene Ontology

Molecular Function

• GO:0004483 mRNA (nucleoside-2'-O-)-methyltransferase activity

External links

• PDB: RCSB:6wks, PDBe:6wks, PDBj:6wks
• PDBsum: 6wks
• PubMed: 32709886
• UniProt: P0DTD1|R1AB_SARS2 Replicase polyprotein 1ab (Gene Name=rep)