Structure of PDB 7pwo Chain A2

Receptor sequence

>7pwoA2 (length=250) Species: 184922 (Giardia lamblia ATCC 50803)

MGRRIRVQRKGAGSVFRAHVARRLGAAKLRANDFAERTGSVRGVVKAILH
DRGRGAPLARVQFPNMRGEGRVNELFIAPEGMYTGQEVFSGNKATLHIGN
ILPVGNIPEGTYVCNVEEKPMDRGCLARASGTYCLVVAHNMETNKTRIRL
PSGQKKLISSKARAMIGLVAGGGRTDKPLLKAGNAYHKYKAKRNCWPVVR
GVAMNPVDHPHGGGNHQHIGVSSCVPRNAVPGQKVGLIAARRTGCSGGKR

3D structure

• PDB: 7pwo Cryo-EM structure of the ancient eukaryotic ribosome from the human parasite Giardia lamblia.
• Chain: A2
• Resolution: 2.75 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A2	M1 G2 R3 R4 V7 Q8 R9 G11 V15 A18 H19 V20 R23 G25 D33 F34 H50 R54 R67 G70 R71 E118 K119 M121 R123 C125 L126 A127 R128 A129 S130 G131 L150 S152 Q154 R174 K177 L179 K181 A182 G183 H187 K188 R193 W196 P197 V198 V199 R200 G201 V202 A203 M204 N205 V207 D208 H211 G214 N215 H216 G220 V221 S222 S223 C224 P226 R227 N228 V230 P231 G232 Q233 K234 V235 G236 I238 A239 A240 R241 R242 T243 G244 C245	M1 G2 R3 R4 V7 Q8 R9 G11 V15 A18 H19 V20 R23 G25 D33 F34 H50 R54 R67 G70 R71 E118 K119 M121 R123 C125 L126 A127 R128 A129 S130 G131 L150 S152 Q154 R174 K177 L179 K181 A182 G183 H187 K188 R193 W196 P197 V198 V199 R200 G201 V202 A203 M204 N205 V207 D208 H211 G214 N215 H216 G220 V221 S222 S223 C224 P226 R227 N228 V230 P231 G232 Q233 K234 V235 G236 I238 A239 A240 R241 R242 T243 G244 C245
BS02	rna	A2	G247 G248 K249 R250	G247 G248 K249 R250

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:0022626 cytosolic ribosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7pwo, PDBe:7pwo, PDBj:7pwo
• PDBsum: 7pwo
• PubMed: 35100413
• UniProt: E2RTN4