Structure of PDB 9bn9 Chain A

Receptor sequence

>9bn9A (length=431) Species: 83333 (Escherichia coli K-12)

ADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGLDKLPEAV
ERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCR
EAQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDE
CELYVLELSSFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLR
IYENAKVCVVNADDALTMPIRRCVSFGVNMGDYHLNHETWLRVKGEKVLN
VKEMKLSGQHNYTNALAALALADAAGLPRASSLKALTTFTGLPHRFEVVL
EHNGVRWINDSKATNVGSTEAALNGLHVDGTLHLLLGGDGKSADFSPLAR
YLNGDNVRLYCFGRDGAQLAALRPEVAEQTETMEQAMRLLAPRVQPGDMV
LLSPACASLDQFKNFEQRGNEFARLAKELGG

3D structure

• PDB: 9bn9 Crystal Structure of UDP-N-acetylmuramoylalanine--D-glutamate ligase (MurD) from E. coli in complex with UMA and two inhibitor A19 molecules
• Chain: A
• Resolution: 1.23 Å

Enzymatic activity

• Enzyme Commision number: 6.3.2.9: UDP-N-acetylmuramoyl-L-alanine--D-glutamate ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	UMA	A	L15 T16 D35 T36 R37 P72 G73 G137 N138 G140 F161 H183	L15 T16 D35 T36 R37 P72 G73 G137 N138 G140 F161 H183
BS02	A1AQS	A	Q170 A171 V172 N204 K206	Q170 A171 V172 N204 K206
BS03	A1AQS	A	F189 Q192 Q193	F189 Q192 Q193

Gene Ontology

Molecular Function

• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0008764 UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
• GO:0016874 ligase activity
• GO:0016881 acid-amino acid ligase activity
• GO:0042802 identical protein binding

Biological Process

• GO:0008360 regulation of cell shape
• GO:0009058 biosynthetic process
• GO:0009252 peptidoglycan biosynthetic process
• GO:0051301 cell division
• GO:0071555 cell wall organization

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:9bn9, PDBe:9bn9, PDBj:9bn9
• PDBsum: 9bn9
• UniProt: P14900|MURD_ECOLI UDP-N-acetylmuramoylalanine--D-glutamate ligase (Gene Name=murD)