Structure of PDB 8ye6 Chain A

Receptor sequence

>8ye6A (length=1359) Species: 301447 (Streptococcus pyogenes serotype M1)

MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGA
LLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHR
LEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKAD
LRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENP
INASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTP
NFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAI
LLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEI
FFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPY
YVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDK
NLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVD
LLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKI
IKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQ
LKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDD
SLTFKEDIQKAQVSDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKNSRERMKRIEEGIKELGSQILKEHPVENTQL
QNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKV
LTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERG
GLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVI
TLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLES
EFVYGDYKVYDVRKMIAKSEQEITAKYFFYSNIMNFFKTEITLANGEIRK
RPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKES
ILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKS
VKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENG
RKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFV
EQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENI
IHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETR
IDLSQLGGD

3D structure

• PDB: 8ye6 Inhibition mechanisms of CRISPR-Cas9 by AcrIIA25.1 and AcrIIA32.
• Chain: A
• Resolution: 2.95 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	M1 S29 K30 I43 K44 E57 T58 A59 T62 K65 R66 R69 R70 R71 Y72 R74 K76 N77 R78 L101 S104 F105 R115 H116 I122 V126 H129 T134 H160 K163 F164 R165 G166 Y325 H328 H329 K336 R340 F352 I363 G366 Q402 R403 T404 F405 N407 G408 F446 R447 I448 Y450 P454 L455 A456 R457 N459 S460 F462 W464 R467 I473 P475 M495 N497 N501 R661 M694 H698 N726 A728 G729 S730 K742 T924 P1090 Q1091 T1102 G1103 G1104 F1105 E1108 I1110 P1112 K1113 R1122 K1123 K1124 Y1131 M1169 E1225 L1226 A1227 Q1272 H1349 Q1350 S1351 Y1356	M1 S29 K30 I43 K44 E57 T58 A59 T62 K65 R66 R69 R70 R71 Y72 R74 K76 N77 R78 L101 S104 F105 R115 H116 I122 V126 H129 T134 H160 K163 F164 R165 G166 Y325 H328 H329 K336 R340 F352 I363 G366 Q402 R403 T404 F405 N407 G408 F446 R447 I448 Y450 P454 L455 A456 R457 N459 S460 F462 W464 R467 I473 P475 M495 N497 N501 R661 M694 H698 N723 A725 G726 S727 K739 T918 P1081 Q1082 T1093 G1094 G1095 F1096 E1099 I1101 P1103 K1104 R1113 K1114 K1115 Y1122 M1160 E1216 L1217 A1218 Q1263 H1340 Q1341 S1342 Y1347

External links

• PDB: RCSB:8ye6, PDBe:8ye6, PDBj:8ye6
• PDBsum: 8ye6
• PubMed: 38842649
• UniProt: Q99ZW2|CAS9_STRP1 CRISPR-associated endonuclease Cas9/Csn1 (Gene Name=cas9)