Structure of PDB 8xol Chain A

Receptor sequence

>8xolA (length=1197) Species: 9606 (Homo sapiens)

EVKPNPLQDANLCSRVFFWWLNPLFKIGHKRRLEEDDMYSVLPEDRSQHL
GEELQGFWDKEVLRAENDAQKPSLTRAIIKCYWKSYLVLGIFTLIEESAK
VIQPIFLGKIINYFENYDPMDSVALNTAYAYATVLTFCTLILAILHHLYF
YHVQCAGMRLRVAMCHMIYRKALRLSNMAMGKTTTGQIVNLLSNDVNKFD
QVTVFLHFLWAGPLQAIAVTALLWMEIGISCLAGMAVLIILLPLQSCFGK
LFSSLRSKTATFTDARIRTMNEVITGIRIIKMYAWEKSFSNLITNLRKKE
ISKILRSSCLRGMNLASFFSASKIIVFVTFTTYVLLGSVITASRVFVAVT
LYGAVRLTVTLFFPSAIERVSEAIVSIRRIQTFLLLDEISQRNRQLPSDG
KKMVHVQDFTAFWDKASETPTLQGLSFTVRPGELLAVVGPVGAGKSSLLS
AVLGELAPSHGLVSVHGRIAYVSQQPWVFSGTLRSNILFGKKYEKERYEK
VIKACALKKDLQLLEDGDLTVIGDRGTTLSGGQKARVNLARAVYQDADIY
LLDDPLSAVDAEVSRHLFELCICQILHEKITILVTHQLQYLKAASQILIL
KDGKMVQKGTYTEGKVGFQAYKNYFRAGAHWIVFIFLILLNTAAQVAYVL
QDWWLSYWANKQSMLNVTVNGGGNVTEKLDLNWYLGIYSGLTVATVLFGI
ARSLLVFYVLVNSSQTLHNKMFESILKAPVLFFDRNPIGRILNRFSKDIG
HLDDLLPLTFLDFIQTLLQVVGVVSVAVAVIPWIAIPLVPLGIIFIFLRR
YFLETSRDVKRLESTTRSPVFSHLSSSLQGLWTIRAYKAEERCQELFDAH
QDLHSEAWFLFLTTSRWFAVRLDAICAMFVIIVAFGSLILAKTLDAGQVG
LALSYALTLMGMFQWCVRQSAEVENMMISVERVIEYTDLEKEAPWEYQKR
PPPAWPHEGVIIFDNVNFMYSPGGPLVLKHLTALIKSQEKVGIVGRTGAG
KSSLISALFRLSEPEGKIWIDKILTTEIGLHDLRKKMSIIPQEPVLFTGT
MRKNLDPFNEHTDEELWNALQEVQLKETIEDLPGKMDTELAESGSNFSVG
QRQLVCLARAILRKNQILIIDEATANVDPRTDELIQKKIREKFAHCTVLT
IAHRLNTIIDSDKIMVLDSGRLKEYDEPYVLLQNKESLFYKMVQQLG

3D structure

• PDB: 8xol The ATP-bound inward-open conformation of ABCC4 reveals asymmetric ATP binding for substrate transport.
• Chain: A
• Resolution: 3.02 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ANP	A	W419 K451 S452 Q480	W413 K445 S446 Q474
BS02	DU0	A	V729 W733 V851 S855 A859	V649 W653 V771 S775 A779
BS03	DU0	A	T338 L341	T332 L335
BS04	DU0	A	W763 G770	W683 G690

Gene Ontology

Molecular Function

• GO:0001409 guanine nucleotide transmembrane transporter activity
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0008559 ABC-type xenobiotic transporter activity
• GO:0015132 prostaglandin transmembrane transporter activity
• GO:0015143 urate transmembrane transporter activity
• GO:0015216 purine nucleotide transmembrane transporter activity
• GO:0015431 ABC-type glutathione S-conjugate transporter activity
• GO:0015432 ABC-type bile acid transporter activity
• GO:0015562 efflux transmembrane transporter activity
• GO:0016404 15-hydroxyprostaglandin dehydrogenase (NAD+) activity
• GO:0034634 glutathione transmembrane transporter activity
• GO:0042626 ATPase-coupled transmembrane transporter activity
• GO:0042910 xenobiotic transmembrane transporter activity
• GO:0043225 ATPase-coupled inorganic anion transmembrane transporter activity
• GO:0140359 ABC-type transporter activity

Biological Process

• GO:0002576 platelet degranulation
• GO:0006805 xenobiotic metabolic process
• GO:0006855 xenobiotic transmembrane transport
• GO:0006869 lipid transport
• GO:0015721 bile acid and bile salt transport
• GO:0015732 prostaglandin transport
• GO:0015747 urate transport
• GO:0032310 prostaglandin secretion
• GO:0034775 glutathione transmembrane transport
• GO:0055085 transmembrane transport
• GO:0060271 cilium assembly
• GO:0070730 cAMP transport
• GO:0071716 leukotriene transport
• GO:0098656 monoatomic anion transmembrane transport
• GO:0140115 export across plasma membrane
• GO:0150104 transport across blood-brain barrier
• GO:1903790 guanine nucleotide transmembrane transport

Cellular Component

• GO:0005730 nucleolus
• GO:0005794 Golgi apparatus
• GO:0005886 plasma membrane
• GO:0016020 membrane
• GO:0016323 basolateral plasma membrane
• GO:0016324 apical plasma membrane
• GO:0031088 platelet dense granule membrane
• GO:0098591 external side of apical plasma membrane

External links

• PDB: RCSB:8xol, PDBe:8xol, PDBj:8xol
• PDBsum: 8xol
• PubMed: 38886124
• UniProt: O15439|MRP4_HUMAN ATP-binding cassette sub-family C member 4 (Gene Name=ABCC4)