Structure of PDB 8xfr Chain A

Receptor sequence

>8xfrA (length=480) Species: 1328314 (Azotobacter chroococcum NCIMB 8003)

MDFDVKDFGAKGDGKSDDTEAIQAAIDAAYEAGGGTVRLSAGEYRVSGGD
EASDGALMIKSNVYMDGAGMGETVIKLVDGWDQKLTGIIRSKNGEKTHDY
GIRDLTLDGNQDNTEGEVDGFYTGYIPREDGADYNVTAERVEIREVSRYG
FDPHEQTINLTIRDSVAHNNGKDGFVGDFQIDSTFENNVSHDNGRHGFNI
VTSSHDILLRDNVAYGNGANGLVVQRGSEDIAHPYNIQIEGGAYHDNGAE
GVLIKMTSNASLQGAEIYGNDAAGVRVRGVDGMQLLDNDIHDNAQGGGKA
EIVLEDYDDRDGVSGNYYETLNATVQGNRVAGAAQLLSSEGRDLLDGAAG
NDLLDGGAGRDTLSGGGGADTFRFADRQDSFRNYEGDTSRVDDIVDFTPG
ADLIDLSGLGYSGLGDGYNGTLALLLNEDGTKTYLKDRQADAQGNHFEIA
LDGNLVDSLSATDIAFDATQLELLGTTDLQ

3D structure

• PDB: 8xfr Structural basis for the minimal bifunctional alginate epimerase AlgE3 from Azotobacter chroococcum.
• Chain: A
• Resolution: 2.0 Å

Enzymatic activity

• Enzyme Commision number: 5.1.3.37: mannuronan 5-epimerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	BEM	A	S53 N93	S53 N93
BS02	BEM	A	S53 T86 R90 N93	S53 T86 R90 N93
BS03	BEM	A	Y122 P127 R128 H154	Y122 P127 R128 H154
BS04	BEM	A	D178 E229	D178 E229
BS05	CA	A	S91 K92 E95 T97 G124 D133	S91 K92 E95 T97 G124 D133
BS06	CA	A	S339 G341 D343 G356 A358 D361	S339 G341 D343 G356 A358 D361
BS07	CA	A	G357 A358 G359 D361 D379 D392	G357 A358 G359 D361 D379 D392
BS08	CA	A	A348 G350 D352 G365 G367 D370	A348 G350 D352 G365 G367 D370
BS09	CA	A	G366 G367 G368 D370 D402	G366 G367 G368 D370 D402
BS10	CA	A	Q111 T114	Q111 T114
BS11	CA	A	D376 D379	D376 D379

Gene Ontology

Molecular Function

• GO:0005509 calcium ion binding
• GO:0016829 lyase activity
• GO:0016853 isomerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0042121 alginic acid biosynthetic process

External links

• PDB: RCSB:8xfr, PDBe:8xfr, PDBj:8xfr
• PDBsum: 8xfr
• PubMed: 38649293
• UniProt: A0A0C4WKK2