Structure of PDB 8xcu Chain A

Receptor sequence

>8xcuA (length=417) Species: 49899 (Thermococcus profundus)

EIDPFEMAVKQLERAAQYMDISEEALEWLKKPMRIVEVSVPIEMDDGSVK
VFTGFRVQHNWARGPTKGGIRWHPAETLSTVKALATWMTWKVAVVDLPYG
GGKGGIIVNPKELSEREQERLARAYIRAVYDVIGPWTDIPAPDVYTNPKI
MGWMMDEYETIMRRKGPAFGVITGKPLSIGGSLGRGTATAQGAIFTIREA
AKALGIDLKGKKIAVQGYGNAGYYTAKLAKEQLGMTVVAVSDSRGGIYNP
DGLDPDEVLKWKREHGSVKDFPGATNITNEELLELEVDVLAPAAIEEVIT
EKNADNIKAKIVAEVANGPVTPEADDILREKGILQIPDFLCNAGGVTVSY
FEWVQNINGYYWTEEEVREKLDKKMTKAFWEVYNTHKDKNIHMRDAAYVV
AVSRVYQAMKDRGWVKK

3D structure

• PDB: 8xcu CryoEM-sampling of metastable conformations appearing in cofactor-ligand association and catalysis of glutamate dehydrogenase
• Chain: A
• Resolution: 2.83 Å

Enzymatic activity

• Enzyme Commision number: 1.4.1.3: glutamate dehydrogenase [NAD(P)(+)].

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	NDP	A	T191 Y220 G221 N222 A223 D244 S245 K264 A296 I297 A318 N319	T189 Y218 G219 N220 A221 D242 S243 K262 A294 I295 A316 N317
BS02	GGL	A	K69 M90 K93 A143 P144 S351	K67 M88 K91 A141 P142 S349

Gene Ontology

Molecular Function

• GO:0004352 glutamate dehydrogenase (NAD+) activity
• GO:0004353 glutamate dehydrogenase [NAD(P)+] activity
• GO:0004354 glutamate dehydrogenase (NADP+) activity
• GO:0016491 oxidoreductase activity
• GO:0016639 oxidoreductase activity, acting on the CH-NH2 group of donors, NAD or NADP as acceptor

Biological Process

• GO:0006520 amino acid metabolic process
• GO:0006538 glutamate catabolic process

External links

• PDB: RCSB:8xcu, PDBe:8xcu, PDBj:8xcu
• PDBsum: 8xcu
• PubMed: 38750092
• UniProt: O74024|DHE3_THEPR Glutamate dehydrogenase (Gene Name=gdhA)