Structure of PDB 8x90 Chain A

Receptor sequence

>8x90A (length=1377) Species: 9606 (Homo sapiens)

VVRKYAKKITEWPPFEYMILATIIANCIVLALEQHLPDDDKTPMSERLDD
TEPYFIGIFCFEAGIKIIALGFSYLRNGWNVMDFVVVLTGILATVGTEFD
LRTLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMIPLLQIGLLLFFAILIF
AIIGLEFYMGKFHTTCFEEGTDDIQGESPAPCGTEEPARTCPNGTKCQPY
WEGPNNGITQFDNILFAVLTVFQCITMEGWTDLLYNSNDASGNTWNWLYF
IPLIIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELN
GYMEWISKAEEVILASTFFHKKERRMRFYIRRMVKTQAFYWTVLSLVALN
TLCVAIVHYNQPEWLSDFLYYAEFIFLGLFMSEMFIKMYGLGTRPYFHSS
FNCFDCGVIIGSIFEVIWAVIKPGTSFGISVLRALRLLRIFKVTKYWASL
RNLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFDEGTPPTN
FDTFPAAIMTVFQILTGEDWNEVMYDGIKSQGGVQGGMVFSIYFIVLTLF
GNYTLLNVFLAIAVDNLANAQELTKDEQEEEEAANQKLALQKAKEVAEVS
NQKPAKSVWEQRTSEMRKQNLLASNPLRRLCHYILNLRYFEMCILMVIAM
SSIALAAEDPVQPNAPRNNVLRYFDYVFTGVFTFEMVIKMIDLGLVLHQG
AYFRDLWNILDFIVVSGALVAFAFTGNSKGKDINTIKSLRVLRVLRPLKT
IKRLPKLKAVFDCVVNSLKNVFNILIVYMLFMFIFAVVAVQLFKGKFFHC
TDESKEFEKDCRGKYLLYEKNEVKARDREWKKYEFHYDNVLWALLTLFTV
STGEGWPQVLKHSVDATFENQGPSPGYRMEMSIFYVVYFVVFPFFFVNIF
VALIIITFQEQGDKMMEEYSLEKNERACIDFAISAKPLTRHMPQNKQSFQ
YRMWQFVVSPPFEYTIMAMIALNTIVLMMKFYGASVAYENALRVFNIVFT
SLFSLECVLKVMAFGILNYFRDAWNIFDFVTVLGSITDILVTEFGNNFIN
LSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFFIY
AIIGMQVFGNIGIDVEDEDSDEDEFQITEHNNFRTFFQALMLLFRSATGE
AWHNIMLSCLSGKPCDKNSGILTRECGNEFAYFYFVSFIFLCSFLMLNLF
VAVIMDNFEYLTRDSSILGPHHLDEYVRVWAEYDPAAWGRMPYLDMYQML
RHMSPPLGLGKKCPARVAYKRLLRMDLPVADDNTVHFNSTLMALIRTALD
IKIAKGGADKQQMDAELRKEMMAIWPNLSQKTLDLLVTPHKSTDLTVGKI
YAAMMIMEYYRQSKAKKLQAMREEQDR

3D structure

• PDB: 8x90 Structural basis for different omega-agatoxin IVA sensitivities of the P-type and Q-type Ca v 2.1 channels.
• Chain: A
• Resolution: 2.95 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	PS1	A	E318 F349 L352 E1459 V1502 A1752 G1754 C1797 M1801	E228 F259 L262 E854 V897 A1147 G1149 C1192 M1196
BS02	CLR	A	F1499 V1502 S1798	F894 V897 S1193
BS03	PT5	A	S548 F550 K591 W596 L599 V603 F1500	S399 F401 K442 W447 L450 V454 F895
BS04	CA	A	E318 E667 E1459	E228 E518 E854
BS05	Y01	A	F621 I657 M658 M1484 I1488	F472 I508 M509 M879 I883
BS06	CLR	A	N303 M1583 F1586	N213 M978 F981
BS07	Y01	A	I505 H507 N509	I356 H358 N360
BS08	Y01	A	I1258 A1261 R1272 F1741 F1742	I653 A656 R667 F1136 F1137
BS09	CLR	A	R1272 V1275 F1279	R667 V670 F674
BS10	CLR	A	F1785 F1788	F1180 F1183

Gene Ontology

Molecular Function

• GO:0001540 amyloid-beta binding
• GO:0005216 monoatomic ion channel activity
• GO:0005245 voltage-gated calcium channel activity
• GO:0005262 calcium channel activity
• GO:0005515 protein binding
• GO:0008331 high voltage-gated calcium channel activity
• GO:0019905 syntaxin binding
• GO:0046872 metal ion binding

Biological Process

• GO:0006811 monoatomic ion transport
• GO:0006816 calcium ion transport
• GO:0007204 positive regulation of cytosolic calcium ion concentration
• GO:0007268 chemical synaptic transmission
• GO:0050804 modulation of chemical synaptic transmission
• GO:0055085 transmembrane transport
• GO:0070588 calcium ion transmembrane transport
• GO:0098703 calcium ion import across plasma membrane
• GO:1904645 response to amyloid-beta
• GO:1904646 cellular response to amyloid-beta

Cellular Component

• GO:0005634 nucleus
• GO:0005737 cytoplasm
• GO:0005886 plasma membrane
• GO:0005891 voltage-gated calcium channel complex
• GO:0016020 membrane
• GO:0034702 monoatomic ion channel complex
• GO:0042995 cell projection
• GO:0043025 neuronal cell body
• GO:0045202 synapse

External links

• PDB: RCSB:8x90, PDBe:8x90, PDBj:8x90
• PDBsum: 8x90
• PubMed: 38443561
• UniProt: O00555|CAC1A_HUMAN Voltage-dependent P/Q-type calcium channel subunit alpha-1A (Gene Name=CACNA1A)