Structure of PDB 8wpp Chain A

Receptor sequence

>8wppA (length=1006) Species: 10244 (Monkeypox virus)

MDVRCINWFESHGENRFLYLKSRCRNGETVFIRFPHYFYYVVTDEIYQSL
SPPPFNARPMGKMRTIDIDETISYNLDIKDRKCSVADMWLIEEPKKRSIQ
NATMDEFFNISWFYISNGISPDGCYSLDEQYLTKINNGCYHCDDPRNCFA
KEIPRFDIPRSYLFLDIECHFDKKFPSVFINPISHTSYCYIDLSGKRLLF
TLINEEMLTEQEIQEAVDRGCLRIQSLMEMDYERELVLCSEIVLLRIAKQ
LLELTFDYVVTFNGHNFDLRYITNRLELLTGEKIIFRSPDKKEAVHLCIY
ERNQSSHKGVCGMANTTFHVNNNNGTIFFDLYSFIQKSEKLDSYKLDSIS
KNAFSCMGKVLNRGVREMTFIGDDTTDAKGKADTFAKVLTTGNYVTVDED
IICKVIRKDILENGFKVVLSCPTLPNDIYKLSFGKDDIDLAQMYKDYNLN
IALDMARYCIHDACLCQYLWEYYGVETKTDAGAATYVLPQSMVFEYRAST
IIKGPLLKLLLETKTILVRSETKQKFPYEGGKVFAPKQKMFSNNVLIFDY
NSLYPNVCIFGNLSPETLVGVVVSTNRLEEEINNQLLLQKYPPPRYITVH
CEPRLPNLISEIAIFDRSIEGTIPRLLRTFLAERARYKKMLKQATSSTEK
AIYDSMQYTYKIVANSVYGLMGFRNSALYSYASAKSCTSIGRRMILYLES
VLNGAELSNGMLRFANTLSNPFYMDDRDINPIVKTSLPIDYRFRFRSVYG
DTDSVFTEIDSQDVDKSIEIAKELERLINSRVLFNNFKIEFEAVYKNLIM
QSKKKYTTMKYSASSNSKSVPERINKGTSETRRDVSKFHKNMIKTYKTRL
SEMLSEGRMNSNQVCIDILRSLETDLRSEFDSRSSPLELFMLSRMHHSNY
KSADNPNMYLVTEYNKNNPETIELGERYYFAYICPANVPWTKKLVNIKTY
ETIIDRSFKLGSNQRIFYEVYFKRLTSEIVNLLDNKVLCISFFQRMFGSR
PTFYEA

3D structure

• PDB: 8wpp Structural insights into the assembly and mechanism of monkeypox virus DNA polymerase complex F8-A22-E4-H5
• Chain: A
• Resolution: 3.1 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	D751 D753 K804 Y806 K826 G827 T831 R832 R833 D834 S893 R894 M895 H897 Y900 K901 N905 R927 R1000	D751 D753 K804 Y806 K826 G827 T831 R832 R833 D834 S893 R894 M895 H897 Y900 K901 N905 R927 R1000
BS02	dna	A	F108 H307 Y496 R497 A498 S499 T500 K503 Y528 E529 G530 V533 Y668 G669 G672 F673 N675 S802 K803 K804 K805 N946 I947 K948 R974 Y1004	F108 H307 Y496 R497 A498 S499 T500 K503 Y528 E529 G530 V533 Y668 G669 G672 F673 N675 S802 K803 K804 K805 N946 I947 K948 R974 Y1004

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0016787 hydrolase activity

Biological Process

• GO:0006260 DNA replication
• GO:0006261 DNA-templated DNA replication
• GO:0006310 DNA recombination
• GO:0039693 viral DNA genome replication

External links

• PDB: RCSB:8wpp, PDBe:8wpp, PDBj:8wpp
• PDBsum: 8wpp
• PubMed: 37995690
• UniProt: A0A7H0DN44|DPOL_MONPV DNA polymerase (Gene Name=OPG071)