Structure of PDB 8wpf Chain A

Receptor sequence

>8wpfA (length=1006) Species: 10244 (Monkeypox virus) [Search protein sequence]

MDVRCINWFESHGENRFLYLKSRCRNGETVFIRFPHYFYYVVTDEIYQSL
SPPPFNARPMGKMRTIDIDETISYNLDIKDRKCSVADMWLIEEPKKRSIQ
NATMDEFFNISWFYISNGISPDGCYSLDEQYLTKINNGCYHCDDPRNCFA
KEIPRFDIPRSYLFLDIECHFDKKFPSVFINPISHTSYCYIDLSGKRLLF
TLINEEMLTEQEIQEAVDRGCLRIQSLMEMDYERELVLCSEIVLLRIAKQ
LLELTFDYVVTFNGHNFDLRYITNRLELLTGEKIIFRSPDKKEAVHLCIY
ERNQSSHKGVCGMANTTFHVNNNNGTIFFDLYSFIQKSEKLDSYKLDSIS
KNAFSCMGKVLNRGVREMTFIGDDTTDAKGKADTFAKVLTTGNYVTVDED
IICKVIRKDILENGFKVVLSCPTLPNDIYKLSFGKDDIDLAQMYKDYNLN
IALDMARYCIHDACLCQYLWEYYGVETKTDAGAATYVLPQSMVFEYRAST
IIKGPLLKLLLETKTILVRSETKQKFPYEGGKVFAPKQKMFSNNVLIFDY
NSLYPNVCIFGNLSPETLVGVVVSTNRLEEEINNQLLLQKYPPPRYITVH
CEPRLPNLISEIAIFDRSIEGTIPRLLRTFLAERARYKKMLKQATSSTEK
AIYDSMQYTYKIVANSVYGLMGFRNSALYSYASAKSCTSIGRRMILYLES
VLNGAELSNGMLRFANTLSNPFYMDDRDINPIVKTSLPIDYRFRFRSVYG
DTDSVFTEIDSQDVDKSIEIAKELERLINSRVLFNNFKIEFEAVYKNLIM
QSKKKYTTMKYSASSNSKSVPERINKGTSETRRDVSKFHKNMIKTYKTRL
SEMLSEGRMNSNQVCIDILRSLETDLRSEFDSRSSPLELFMLSRMHHSNY
KSADNPNMYLVTEYNKNNPETIELGERYYFAYICPANVPWTKKLVNIKTY
ETIIDRSFKLGSNQRIFYEVYFKRLTSEIVNLLDNKVLCISFFQRMFGSR
PTFYEA

3D structure

PDB

8wpf Structural insights into the assembly and mechanism of monkeypox virus DNA polymerase complex F8-A22-E4-H5

Chain

Resolution

3.0 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	K340 D751 K804 Y806 K826 G827 T831 R832 R833 D834 R894 H897 Y900 K901 N907 R927 K943 R1000	K340 D751 K804 Y806 K826 G827 T831 R832 R833 D834 R894 H897 Y900 K901 N907 R927 K943 R1000
BS02	dna	A	H12 K96 F108 H307 Y496 R497 A498 S499 T500 K503 K525 Y528 E529 G530 V533 N665 S666 Y668 G669 L670 G672 F673 N675 S802 K803 K804 K805 N946 I947 K948 V970 R974 S977 Y1004	H12 K96 F108 H307 Y496 R497 A498 S499 T500 K503 K525 Y528 E529 G530 V533 N665 S666 Y668 G669 L670 G672 F673 N675 S802 K803 K804 K805 N946 I947 K948 V970 R974 S977 Y1004
BS03	D3T	A	D549 Y550 S552 Y554 R634 K661 I662 N665 Y668 D753	D549 Y550 S552 Y554 R634 K661 I662 N665 Y668 D753
BS04	MG	A	D549 Y550 D753	D549 Y550 D753

Gene Ontology

	Molecular Function
GO:0000166	nucleotide binding
GO:0003676	nucleic acid binding
GO:0003677	DNA binding
GO:0003887	DNA-directed DNA polymerase activity
GO:0016787	hydrolase activity

	Biological Process
GO:0006260	DNA replication
GO:0006261	DNA-templated DNA replication
GO:0006310	DNA recombination
GO:0039693	viral DNA genome replication

View graph for
Molecular Function

View graph for
Biological Process

External links

PDB	RCSB:8wpf, PDBe:8wpf, PDBj:8wpf
PDBsum	8wpf
PubMed	37995690
UniProt	A0A7H0DN44\|DPOL_MONPV DNA polymerase (Gene Name=OPG071)

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