Structure of PDB 8woo Chain A

Receptor sequence

>8wooA (length=1217) Species: 3702 (Arabidopsis thaliana)

EQSLPFFKLFSFADKFDYLLMFVGSLGAIVHGSSMPVFFLLFGQMVNGFG
KNQMDLHQMVHEVSRYSLYFVYLGLVVCFSSYAEIACWMYSGERQVAALR
KKYLEAVLKQDVGFFDTDARTGDIVFSVSTDTLLVQDAISEKVGNFIHYL
STFLAGLVVGFVSAWKLALLSVAVIPGIAFAGGLYAYTLTGITSKSRESY
ANAGVIAEQAIAQVRTVYSYVGESKALNAYSDAIQYTLKLGYKAGMAKGL
GLGCTYGIACMSWALVFWYAGVFIRNGQTDGGKAFTAIFSAIVGGMSLGQ
SFSNLGAFSKGKAAGYKLMEIINQRPTIIQDPLDGKCLDQVHGNIEFKDV
TFSYPSRPDVMIFRNFNIFFPSGKTVAVVGGSGSGKSTVVSLIERFYDPN
SGQILLDGVEIKTLQLKFLREQIGLVNQEPALFATTILENILYGKPDATM
VEVEAAASAANAHSFITLLPKGYDTQVGERGVQLSGGQKQRIAIARAMLK
DPKILLLDEATSALDASSESIVQEALDRVMVGRTTVVVAHRLCTIRNVDS
IAVIQQGQVVETGTHEELIAKSGAYASLIRFQEMVSTRLSHSLSTKSLSL
RSGSLRNLSYSYSTGADGRIEMISNAETDRKTRAPENYFYRLLKLNSPEW
PYSIMGAVGSILSGFIGPTFAIVMSNMIEVFYYTDYDSMERKTKEYVFIY
IGAGLYAVGAYLIQHYFFSIMGENLTTRVRRMMLSAILRNEVGWFDEDEH
NSSLIAARLATDAADVKSAIAERISVILQNMTSLLTSFIVAFIVEWRVSL
LILGTFPLLVLANFAQQLSLKGFAGDTAKAHAKTSMIAGEGVSNIRTVAA
FNAQSKILSLFCHELRVPQKRSLYRSQTSGFLFGLSQLALYGSEALILWY
GAHLVSKGVSTFSKVIKVFVVLVITANSVAETVSLAPEIIRGGEAVGSVF
SVLDRQTRIDPDDADADPVETIRGDIEFRHVDFAYPSRPDVMVFRDFNLR
IRAGHSQALVGASGSGKSSVIAMIERFYDPLAGKVMIDGKDIRRLNLKSL
RLKIGLVQQEPALFAATIFDNIAYGKDGATESEVIDAARAANAHGFISGL
PEGYKTPVGERGVQLSGGQKQRIAIARAVLKNPTVLLLDEATSALDAESE
CVLQEALERLMRGRTTVVVAHRLSTIRGVDCIGVIQDGRIVEQGSHSELV
SRPEGAYSRLLQLQTHR

3D structure

• PDB: 8woo Structure and function of the ABC transporter ABCB19 in brassinosteroid export
• Chain: A
• Resolution: 3.9 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ANP	A	D136 Y374 S402 G403 G405 K406 S407 T408 E529	D116 Y354 S382 G383 G385 K386 S387 T388 E509
BS02	ANP	A	D780 Y1019 S1021 R1022 G1048 S1049 G1050 K1051 S1052 A1175	D746 Y985 S987 R988 G1014 S1015 G1016 K1017 S1018 A1141
BS03	BLD	A	Y276 F309 F704 V954 V957	Y256 F289 F670 V920 V923

Gene Ontology

Molecular Function

• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0010329 auxin efflux transmembrane transporter activity
• GO:0016887 ATP hydrolysis activity
• GO:0140359 ABC-type transporter activity

Biological Process

• GO:0008361 regulation of cell size
• GO:0009637 response to blue light
• GO:0009639 response to red or far red light
• GO:0009640 photomorphogenesis
• GO:0009733 response to auxin
• GO:0009734 auxin-activated signaling pathway
• GO:0009926 auxin polar transport
• GO:0009958 positive gravitropism
• GO:0010218 response to far red light
• GO:0010315 auxin export across the plasma membrane
• GO:0010540 basipetal auxin transport
• GO:0010541 acropetal auxin transport
• GO:0043481 anthocyanin accumulation in tissues in response to UV light
• GO:0048364 root development
• GO:0048443 stamen development
• GO:0048527 lateral root development
• GO:0055085 transmembrane transport
• GO:0060918 auxin transport
• GO:0090691 formation of plant organ boundary

Cellular Component

• GO:0005829 cytosol
• GO:0005886 plasma membrane
• GO:0016020 membrane

External links

• PDB: RCSB:8woo, PDBe:8woo, PDBj:8woo
• PDBsum: 8woo
• PubMed: 38513023
• UniProt: Q9LJX0|AB19B_ARATH ABC transporter B family member 19 (Gene Name=ABCB19)