Structure of PDB 8wmi Chain A

Receptor sequence

>8wmiA (length=1237) Species: 45657 (Desulfonema ishimotonii)

MTTTMKISIEFLEPFRMTKWQESTRRNKNNKEFVRGQAFARWHRNKKDNT
KGRPYITGTLLRSAVIRSAENLLTLSDGKISEKTCCPGKFDTEDKDRLLQ
LRQRSTLRWTDKNPCPDNAETYCPFCELLGRSFRIHFGNLSLPGKPDFDG
PKAIGSQRVLNRVDFKSGKAHDFFKAYEVDHTRFPRFEGEITIDNKVSAE
ARKLLCDSLKFTDRLCGALCVIRFDNLAEKTAEQIISILDDNKKTEYTRL
LADAIRSLRRSSKLVAGLPKDHDGKDDHYLWDIGTIRQILTTSADTKELK
NAGKWREFCEKLGEALYLKSKSVLKETVVCGELVAKTPFFFGAIDEDAKQ
TALQVLLTPDNKYRLPRSAVRGILRRDLQTYFDSPCNAELGGRPCMCKTC
RIMRGITVMDARSEYNAPPEIRHRTRINPFTGTVAEGALFNMEVAPEGIV
FPFQLRYRGSEDGLPDALKTVLKWWAEGQAFMSGAASTGKGRFRMENAKY
ETLDLSDENQRNDYLKNWGWRDEKGLEELKKRLNSGLPEPGNYRDPKWHE
INVSIEMASPFINGDPIRAAVDKRGTAVVTFVKYKAEGEEAKPVCAYKAE
SFRGVIRSAVARIHMEDGVPLTELTHSDCECLLCQIFGSEYEAGKIRFED
LVFESDPEPVTFDHVAIDRFTGGAADKKKFDDSPLPGSPARPLMLKGSFW
IRRDVLEDEEYCKALGKALADVNNGLYPLGGKSAIGYGQVKSLGIKGDDK
RISRLMNAVPEKPKTDAEVRIEAEKVYYPHYFVEPHKKVEREEKPCGHQK
FHEGRLTGKIRCKLITKTPLIVPDTSNDDFFRPYHKSYAFFRLHKQIMIP
GSELRGMVSSVYETVTNSCFRIFDETKRLSWRMDADQDFLPGRVTADGKH
IQKFSETEEYEIPEKARIKYKELLRVYNNNPQADLVYFKHNEKYVEDIVP
VRISRTVDDRMIGKRMSADLRPCHGGLCPACRLFGTGSYKGRVRFGFASL
ENDPEWLIPGKNPGDPFHGGPVMLSLLERPRPTWSIPGSDNKFKVPGRKF
YVHHHAWKTIKDGNHPTTGKAIEQSPNNRTVEALAGGNSFSFEIAFENLK
EWELGLLIHSLQLEKGLAHKLGMAKSMGFGSVEIDVESVRLRKDWKQWRN
GNSEIPNWLGKGFAKLKEWFRDELDFIENLKKLLWFPEGDQAPRVCYPML
RKKDDPNGNSGYEELKDGEFKKEDRQKKLTTPWTPWA

3D structure

• PDB: 8wmi Structural basis of negative regulation of CRISPR-Cas7-11 by TPR-CHAT.
• Chain: A
• Resolution: 3.53 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	R35 Q37 F39 R41 H43 Y55 T57 T59 R62 R67 F90 D91 T92 E93 L101 R102 Q103 R104 R131 F146 H149 F150 N152 V172 L173 N174 R175 V176 K182 A183 F186 F187 R227 G419 S445 R448 L467 G468 R481 H500 R501 T502 I504 V511 L516 F517 G641 K675 G681 R684 S716 D740 H741 V742 A743 I744 R746 G750 A751 K756 F757 A811 P908 T910 R967 R978 Y1245 N1248 Q1250 V1290 R1291 I1292 T1350 G1351 E1392 R1393 R1395 W1398 M1487 A1488 K1489 Y1561 L1564 Y1576	R35 Q37 F39 R41 H43 Y55 T57 T59 R62 R67 F90 D91 T92 E93 L101 R102 Q103 R104 R131 F133 H136 F137 N139 V159 L160 N161 R162 V163 K169 A170 F173 F174 R214 G342 S368 R371 L390 G391 R404 H423 R424 T425 I427 V434 L439 F440 G564 K598 G604 R607 S639 D663 H664 V665 A666 I667 R669 G673 A674 K679 F680 A734 P823 T825 R871 R882 Y927 N930 Q932 V951 R952 I953 T986 G987 E1028 R1029 R1031 W1034 M1123 A1124 K1125 Y1197 L1200 Y1212
BS02	ZN	A	C86 C126	C86 C126
BS03	ZN	A	H703 C708	H626 C631
BS04	ZN	A	C965 C1312 C1342	C869 C973 C978

Gene Ontology

Biological Process

• GO:0051607 defense response to virus

External links

• PDB: RCSB:8wmi, PDBe:8wmi, PDBj:8wmi
• PDBsum: 8wmi
• PubMed: 38735995
• UniProt: A0A401FT36