Structure of PDB 8wm4 Chain A

Receptor sequence

>8wm4A (length=1331) Species: 45657 (Desulfonema ishimotonii)

MTTTMKISIEFLEPFRMTKWQESTRRNKNNKEFVRGQAFARWHRNKKDNT
KGRPYITGTLLRSAVIRSAENLLTLSDGKISEKTCCPGKFDTEDKDRLLQ
LRQRSTLRWTDKNPCPDNAETYCPFCELLGRSFRIHFGNLSLPGKPDFDG
PKAIGSQRVLNRVDFKSGKAHDFFKAYEVDHTRFPRFEGEITIDNKVSAE
ARKLLCDSLKFTDRLCGALCVIRFDNLAEKTAEQIISILDDNKKTEYTRL
LADAIRSLRRSSKLVAGLPKDHDGKDDHYLWDIGVTIRQILTTSADTKEL
KNAGKWREFCEKLGEALYLKSKSVLKETVVCGELVAKTPFFFGAIDEDAK
QTDLQVLLTPDNKYRLPRSAVRGILRRDLQTYFDSPCNAELGGRPCMCKT
CRIMRGITVMDARSEYNAPPEIRHRTRINPFTGTVAEGALFNMEVAPEGI
VFPFQLRYRGSEDGLPDALKTVLKWWAEGQAFMSGAASTGKGRFRMENAK
YETLDLSDENQRNDYLKNWGWRDEKGLEELKKRLNSGLPEPGNYRDPKWH
EINVSIEMASPFINGDPIRAAVDKRGTDVVTFVKYKAEGEEAKPVCAYKA
ESFRGVIRSAVARIHMEDGVPLTELTHSDCECLLCQIFGSEYEAGKIRFE
DLVFESDPEPVTFDHVAIDRFTGGAADKKKFDDSPLPGSPARPLMLKGSF
WIRRDVLEDEEYCKALGKALADVNNGLYPLGGKSAIGYGQVKSLGIKGDD
KRISRLMNAVPEKPKTDAEVRIEAEKVYYPHYFVEPHKKVEREEKPCGHQ
KFHEGRLTGKIRCKLITKTPLIVPDTSNDDFFRPYHKSYAFFRLHKQIMI
PGSELRGMVSSVYETVTNSCFRIFDETKRLSWRMDADQDFLPGRVTADGK
HIQKFSETARVPPENDPDGWECKEGYLHVVGPSKVEFSDKKDWKTIRTND
FKNRKRKNEPVFCCEDDKGNYYTMAKYCETFFFDLKENEEYEIPEKARIK
YKELLRVYNNNPQAVPNVEKLKSGDLVYFKHNEKYVEDIVPVRISRTVDD
RMIGKRMSADLRPCHGDWKGLCPACRLFGTGSYKGRVRFGFASLENDPEW
LIPGKNPGDPFHGGPVMLSLLERPRPTWSIPGSDNKFKVPGRKFYVHHHA
WKTIKDGNHPTTGKAIEQSPNNRTVEALAGGNSFSFEIAFENLKEWELGL
LIHSLQLEKGLAHKLGMAKSMGFGSVEIDVESVRLRKDWKQWRNGNSEIP
NWLGKGFAKLKEWFRDELDFIENLKKLLWFPEGDQAPRVCYPMLRKKDDP
NGNSGYEELKDGEFKKEDRQKKLTTPWTPWA

3D structure

• PDB: 8wm4 Structural basis of negative regulation of CRISPR-Cas7-11 by TPR-CHAT.
• Chain: A
• Resolution: 2.93 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	E13 R35 A38 H43 R53 T57 T59 L60 R62 S63 R67 K89 F90 D91 T92 Q100 R102 Q103 R104 L129 F146 H149 F150 N152 S154 K158 V172 L173 N174 V176 L232 I421 R444 R448 G449 I450 R453 L467 G468 T484 V485 R501 T502 R503 I504 T510 V511 L516 F517 M559 G561 A562 A563 S564 G641 P643 K675 S678 V682 R684 S716 E719 H741 V742 I744 G749 A751 K756 F757 G808 K809 S810 Y863 P908 T910 E949 R951 R967 I968 R978 R1010 K1155 V1156 E1157 F1158 N1195 E1196 V1198 Q1250 V1290 R1291 I1292 S1293 R1294 G1349 T1350 Y1353 K1354 L1391 E1392 R1393 R1395 T1397 W1398 Y1415 R1443 M1487 A1488 K1489 Y1561 L1564 Y1576 K1580	E13 R35 A38 H43 R53 T57 T59 L60 R62 S63 R67 K89 F90 D91 T92 Q100 R102 Q103 R104 L129 F133 H136 F137 N139 S141 K145 V159 L160 N161 V163 L219 I345 R368 R372 G373 I374 R377 L391 G392 T408 V409 R425 T426 R427 I428 T434 V435 L440 F441 M483 G485 A486 A487 S488 G565 P567 K599 S602 V606 R608 S640 E643 H665 V666 I668 G673 A675 K680 F681 G732 K733 S734 Y779 P824 T826 E854 R856 R872 I873 R883 R910 K934 V935 E936 F937 N958 E959 V961 Q1013 V1042 R1043 I1044 S1045 R1046 G1079 T1080 Y1083 K1084 L1121 E1122 R1123 R1125 T1127 W1128 Y1145 R1173 M1217 A1218 K1219 Y1291 L1294 Y1306 K1310
BS02	ZN	A	C86 C126	C86 C126
BS03	ZN	A	H703 C708 C711	H627 C632 C635
BS04	ZN	A	C965 C1312 C1342 C1345	C870 C1064 C1072 C1075

Gene Ontology

Biological Process

• GO:0051607 defense response to virus

External links

• PDB: RCSB:8wm4, PDBe:8wm4, PDBj:8wm4
• PDBsum: 8wm4
• PubMed: 38735995
• UniProt: A0A401FT36