Structure of PDB 8wcs Chain A

Receptor sequence

>8wcsA (length=1044) Species: 32644 (unidentified)

SGKIIEKLDELKKATQESIEQRKKFDYRIGKGLADKLGNEKDSYFEKYLR
KTVGENIPPKILVKPKGGWKTETKGKGKDWGDKITFAAMPQHHYNTLLAM
AFTKAHKVYSDIRGKVEIDAAQYERGLSGLEYLMMSKHLFSGKNSNIKLA
VKKGETEILKEYALNEKLIYTVLDELRNFHSHIFHEPGPVSFKNLYGDEY
KPEKKLTEEEWAIARDWFVNRFNDAKEHKLKTLAKVLEREGTTEEKEDAE
KVIKTISGYSFEYNNCISREALLFIACMFLRKSDAAYFTKKWTGMKKAEG
VFKSTQSFFTDNALKESKSILTLNADLYKYRQILGVLSTMPAMKTDSLKP
FYDFIKINNDSYSEKAEKARSKEEKEKIQAFIIPQRKSSNYTYWFMKYLN
DNKLLDGFRIAYYKTPEDRFMYLIHNSLTEIYKETQRNFVFKVPTIENDN
FCVKKLNVFFQTDIEFNGQKISVQLSVSPDFLMKWVFVLLITGEDSIKNA
ITEKKEKIKDILKKYAEEYYNRCITSNFNEPLMGLEASKVFPSSLTSTVE
IDEKIDKDKILMRISEKYNELTKFDEENKSRKAPWRFASKRKIDIILDYV
HLVYSDRAFDEKKSVDAMRHEALNDMEYMDTFEYLRYYGRYRETEEFKKI
FFEDKKLYFSPILKAMKQLDSLEGVFNFAITGFLNYLKGIQSKVTDENTN
KYGKVFKVTGKSLTSKIGHHSEMFSVNHCVPQELIKLNDIKGYMKWKHET
KDKLWISDFAFIRNVLESRGGFSNTDYLMKEVMPLITFEKNEKGSIKGNT
QMFVALSRNKTNELMLWEIGKYYWKEATGSEFSRLFKGLEKNTGNKITKA
YRFTNPYYTIYQEDLDIKIQRKDKKGKVIVNSPVYTIKIKPKKFDDEYQY
YEQEHIVDYIENYEPKKGIDGHWHFEELNKKIKDELARYLDDIYLLMTVE
KHIVQKDFDKYASLLKEKDSKLPPYYIGFKRNDIALNKVIFDALKHKGSF
SADDLNIYRINVLHQILQPKREKYIIIRKSLIEYCAENKLLKTM

3D structure

• PDB: 8wcs Molecular mechanism for target RNA recognition and cleavage of Cas13h.
• Chain: A
• Resolution: 3.1 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	R36 G38 K39 G40 L41 I92 A96 M97 Q99 H101 Y102 S164 G165 K175 H251 K252 K254 T255 K258 R262 Y286 S291 R292 G317 G323 S327 T368 D369 N382 D383 S386 Q402 I405 K410 Y436 Y499 K500 T502 Q503 R504 N517 V520 K521 K522 N524 S545 D547 K606 P609 S610 S611 M629 R630 E633 K649 W652 R653 K657 H668 R686 L690 D692 Y695 M696 R703 Y704 G706 R707 K771 K774 S779 H787 M790 F791 N794 C796 K820 W822 I823 S824 F826 R830 D843 M846 K847 M850 I853 K860 S862 I863 G865 N866 T867 Q868 F870 V871 S874 R875 K959 K997 K1000 D1001	R28 G30 K31 G32 L33 I84 A88 M89 Q91 H93 Y94 S141 G142 K152 H228 K229 K231 T232 K235 R239 Y263 S268 R269 G294 G300 S304 T345 D346 N359 D360 S363 Q379 I382 K387 Y413 Y432 K433 T435 Q436 R437 N450 V453 K454 K455 N457 S478 D480 K539 P542 S543 S544 M562 R563 E566 K582 W585 R586 K590 H601 R619 L623 D625 Y628 M629 R636 Y637 G639 R640 K704 K707 S712 H720 M723 F724 N727 C729 K753 W755 I756 S757 F759 R763 D776 M779 K780 M783 I786 K793 S795 I796 G798 N799 T800 Q801 F803 V804 S807 R808 K892 K930 K933 D934

External links

• PDB: RCSB:8wcs, PDBe:8wcs, PDBj:8wcs
• PDBsum: 8wcs
• PubMed: 38661236