Structure of PDB 8wce Chain A

Receptor sequence

>8wceA (length=994) Species: 32644 (unidentified)

PQHHYNTLLAMAFTKAHKVYSDIRGKVEIDAAQYETKAKLIEVEYGKDET
RGLSGLEYLMMSKHLFSGKNSNIKLAVKKGETEILKEYALNEKLIYTVLD
ELRNFHSHIFHEPGPVSFKNLYGDEYKPEKKLTEEEWAIARDWFVNRFND
AKEHKLKTLAKVLEREGTTEEKEDAEKVIKTISGYSFEYNNCISREALLF
IACMFLRKSDAAYFTKKWTGMKKAEGVFKSTQSFFTDNALKESKSILTLN
ADLYKYRQILGVLSTMPAMKTDSLKPFYDFIKINNDSYSEKAEKARSKEE
KEKIQAFIIPQRKSSNYTYWFMKYLNDNKLLDGFRIAYYKTPEDRFMYLI
HNGLISQDDLENIEDFKTPDEKLKYLREKGFNLKLKMKQAVGDEKKSLTE
IYKETQRNFVFKVPTIENDNFCVKKLNVFFQTDIEFNGQKISVQLSVSPD
FLMKWVFVLLITGEDSIKNAITEKKEKIKDILKKYAEEYYNRCITSNFNE
PLMGLEASKVFPSSLTSTVEIDEKIDKDKILMRISEKYNELTKFDEENKS
RKAPWRFASKRKIDIILDYVHLVYSDRAFDEKKSVDAMRHEALNDMEYMD
TFEYLRYYGRYRETEEFKKIFFEDKKLYFSPILKAMKQLDSLEGVFNFAI
TGFLNYLKGIQSKVTDENTNKYGKVFKVTGKSLTSKIGHHSEMFSVNHCV
PQELIKLNDIKGYMKWKHETKDKLWISDFAFIRNVLESRGGFSNTDYLMK
EVMPLITFEKNEKGSIKGNTQMFVALSRNKTNELMLWEIGKYYWKEATGS
EFSRLFKGLEKNTGNKITKAYRFTNPYYTIYQEDLDIKIQRKDKKGKVIV
NSPVYTIKIKPKKFDDEYQYYEQEHIVDYIENYEPKKGIDGHWHFEELNK
KIKDELARYLDDIYLLMTVEKHIVQKDFDKYASIALNKVIFDALKHKGSF
SADDLNIYRINVLHQILQPKREKYIIIRKSLIEYCAENKLLKTM

3D structure

• PDB: 8wce Molecular mechanism for target RNA recognition and cleavage of Cas13h.
• Chain: A
• Resolution: 3.09 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	V140 K171 K326 Y351 Q355 G358 D369 Q408 R409 K410 R474 Q503 K521 K522 N524 S545 K606 P609 S610 S611 H668 R686 L690 N691 D692 Y695 M696 K771 S779 H787 N794 C796 K820 L821 W822 I823 S824 F826 R830 M846 K847 M850 P851 I853 F855 K857 S862 G865 N866 T867 Q868 F870 R875 K877 T878 K960	V43 K74 K229 Y254 Q258 G261 D272 Q311 R312 K313 R377 Q406 K424 K425 N427 S448 K509 P512 S513 S514 H571 R589 L593 N594 D595 Y598 M599 K674 S682 H690 N697 C699 K723 L724 W725 I726 S727 F729 R733 M749 K750 M753 P754 I756 F758 K760 S765 G768 N769 T770 Q771 F773 R778 K780 T781 K863
BS02	rna	A	S342 R354 Y436 W652 R653 S656 K657 F699 R703 Y704 L739	S245 R257 Y339 W555 R556 S559 K560 F602 R606 Y607 L642
BS03	MG	A	K959 D962 D963	K862 D865 D866

External links

• PDB: RCSB:8wce, PDBe:8wce, PDBj:8wce
• PDBsum: 8wce
• PubMed: 38661236