Structure of PDB 8uzb Chain A

Receptor sequence

>8uzbA (length=910) Species: 1422 (Geobacillus stearothermophilus) [Search protein sequence]

MRYKIGLAIGITSVGWAVMNLDIPRIEDLGVRIFDRAENPQTGESLALPR
RLARSARRRLRRRKHRLERIRRLVIREGILTKEELDKLFEEKHEIDVWQL
RVEALDRKLNNDELARVLLHLAKRRGFKSNRKSSTMLKHIEGNRAILSSY
RTVGEMIVKDPKFALHKRNKGENYINTIARDDLEREIRLIFSKQREFGDM
SCTEEFENEYITIWASQRPVASKDDIEKKVGFCTFEPKEKRAPKATYTFQ
SFIAWEHINKLRLISPSGARGLTDEERRLLYEQAFQKNKITYHDIRTLLH
LPDDTYFKGIVYDRGESRKQNENIRFLELDAYHQIRKAVDKVYGKGKSSS
FLPIDFDTFGYALTLFKDDADIHSYLRNEYEQNGKRMPNLANKVYDNELI
EELLNLSFTKFGHLSLKALRSILPYMEQGEVYSSACERAGYTFTGPKKKQ
KTMLLPNIQPIANPVVMRALTQARKVVNAIIKKYGSPVSIHIELARDLSQ
TFDERRKTKKEQDENRTRYISRFFANFIREHLKFAESDDKQKVYTVNGRV
TAHLRSRWEFNKNREESDLHHAVDAVIVACTTPSDIAKVTAFYQRREQNE
PHFPQPWPHFADELRARLSKHPKESIKALNLGNYDDQKLESLQPVFVSRM
PKRSVTGAAHRETLRRYVGIDERSGKIQTVVKTKLSKIKLDASGHFPMYG
KESDPRTYEAIRQRLLEHNNDPKKAFQGPLYKPKKNGEPGPVIRTVKIID
TRNQVIPLNDGKTVAYNSNIVRVDVFEKDGKYYCVPVYTMDIMKGILPNK
AIEPNKPYSEWKEMTEDYTFRFSLYPNDLIRIELPREKTVKTAAGEEINV
KDVFVYYKTIDSANGGLELISHDHRFSLRGVGSRTLKRFEKYQVDVLGNI
YKVRGEKRVG

3D structure

PDB

8uzb Accelerated DNA unwinding supercharges genome editing by engineered CRISPR-Cas9

Chain

Resolution

2.63 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	S45 L46 R50 R51 R54 R57 R58 R59 R61 R62 K64 H65 R69 R72 F89 K92 D96 V97 W98 H120 K123 R124 R125 G126 K174 R175 N176 K177 G178 Y181 R187 S223 Q224 R225 P226 V227 K251 E263 H264 R332 H420 Y439 F450 T451 N464 R475 K489 P807 R809 V811 T812 G813 A814 A815 H816 L820 R822 V837 K838 E858 S859 D860 R862 K888 K890 K891 V898 R900 K903 K918 T919 M949 Q1049 Y1057 V1059 R1060 K1063	S45 L46 R50 R51 R54 R57 R58 R59 R61 R62 K64 H65 R69 R72 F89 K92 D96 V97 W98 H120 K123 R124 R125 G126 K167 R168 N169 K170 G171 Y174 R180 S216 Q217 R218 P219 V220 K244 E256 H257 R325 H413 Y432 F443 T444 N457 R468 K482 P651 R653 V655 T656 G657 A658 A659 H660 L664 R666 V681 K682 E702 S703 D704 R706 K732 K734 K735 V742 R744 K747 K762 T763 M793 Q893 Y901 V903 R904 K907
BS02	dna	A	S129 N130 R131 K132 M143 E179 Y181 I233 K236 V237 R248 K267 R269 K315 G316 K374 T416 K417 F418 S440 F450 E818 T819 N1020 R1035 G1036 V1037 G1038 R1040 T1041	S129 N130 R131 K132 M136 E172 Y174 I226 K229 V230 R241 K260 R262 K308 G309 K367 T409 K410 F411 S433 F443 E662 T663 N864 R879 G880 V881 G882 R884 T885
BS03	dna	A	Q41 T907 R908 N909 N925 I926 Y944 R992 T1015 D1017 S1018 A1019	Q41 T751 R752 N753 N769 I770 Y788 R836 T859 D861 S862 A863

Gene Ontology

	Molecular Function
GO:0003677	DNA binding
GO:0003723	RNA binding
GO:0004519	endonuclease activity
GO:0046872	metal ion binding

	Biological Process
GO:0043571	maintenance of CRISPR repeat elements
GO:0051607	defense response to virus

View graph for
Molecular Function

View graph for
Biological Process

External links

PDB	RCSB:8uzb, PDBe:8uzb, PDBj:8uzb
PDBsum	8uzb
PubMed	38781968
UniProt	A0A150MP45

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