Structure of PDB 8uza Chain A

Receptor sequence

>8uzaA (length=910) Species: 1422 (Geobacillus stearothermophilus) [Search protein sequence]

MRYKIGLAIGITSVGWAVMNLDIPRIEDLGVRIFDRAENPQTGESLALPR
RLARSARRRLRRRKHRLERIRRLVIREGILTKEELDKLFEEKHEIDVWQL
RVEALDRKLNNDELARVLLHLAKRRGFKSNRKSSTMLKHIEENRAILSSY
RTVGEMIVKDPKFALHKRNKGENYTNTIARDDLEREIRLIFSKQREFGNM
SCTEEFENEYITIWASQRPVASKDDIEKKVGFCTFEPKEKRAPKATYTFQ
SFIAWEHINKLRLISPSGARGLTDEERRLLYEQAFQKNKITYHDIRTLLH
LPDDTYFKGIVYDRGESRKQNENIRFLELDAYHQIRKAVDKVYGKGKSSS
FLPIDFDTFGYALTLFKDDADIHSYLRNEYEQNGKRMPNLANKVYDNELI
EELLNLSFTKFGHLSLKALRSILPYMEQGEVYSSACERAGYTFTGPKKKQ
KTMLLPNIPPIANPVVMRALTQARKVVNAIIKKYGSPVSIHIELARDLSQ
TFDERRKTKKEQDENRTRYISRFFANFIREHLKFAESDDKQKVYTVNGRV
TAHLRSRWEFNKNREESDLHHAVDAVIVACTTPSDIAKVTAFYQRREQNE
PHFPQPWPHFADELRARLSKHPKESIKALNLGNYDDQKLESLQPVFVSRM
PKRSVTGAAHQETLRRYVGIDERSGKIQTVVKTKLSEIKLDASGHFPMYG
KESDPRTYEAIRQRLLEHNNDPKKAFQEPLYKPKKNGEPGPVIRTVKIID
TKNQVIPLNDGKTVAYNSNIVRVDVFEKDGKYYCVPVYTMDIMKGILPNK
AIEPNKPYSEWKEMTEDYTFRFSLYPNDLIRIELPREKTVKTAAGEEINV
KDVFVYYKTIDSANGGLELISHDHRFSLRGVGSRTLKRFEKYQVDVLGNI
YKVRGEKRVG

3D structure

PDB

8uza Accelerated DNA unwinding supercharges genome editing by engineered CRISPR-Cas9

Chain

Resolution

3.17 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	S45 L46 R50 R51 R57 R58 R59 R61 R62 K64 H65 R71 R72 F89 K92 D96 W98 H120 K123 R124 R125 G126 H173 K174 R175 N176 K177 G178 R187 S223 Q224 R225 V227 K251 E263 H420 Y439 A469 T478 K489 R809 V811 T812 G813 H816 L820 R822 K838 S859 D860 K888 K890 K891 V898 R900 K918 T919 M949 Q1049 Y1057 K1058 V1059 R1060 V1065	S45 L46 R50 R51 R57 R58 R59 R61 R62 K64 H65 R71 R72 F89 K92 D96 W98 H120 K123 R124 R125 G126 H166 K167 R168 N169 K170 G171 R180 S216 Q217 R218 V220 K244 E256 H413 Y432 A462 T471 K482 R653 V655 T656 G657 H660 L664 R666 K682 S703 D704 K732 K734 K735 V742 R744 K762 T763 M793 Q893 Y901 K902 V903 R904 V909
BS02	dna	A	F127 S129 Y181 K236 V237 R248 K267 K315 K374 T416 F418 S440 F450 E818 T819 N1020 R1035 G1036 V1037 G1038 R1040 T1041	F127 S129 Y174 K229 V230 R241 K260 K308 K367 T409 F411 S433 F443 E662 T663 N864 R879 G880 V881 G882 R884 T885
BS03	dna	A	Q41 S830 K832 K908 N909 N925 I926 Y944 N961 T1015 D1017 S1018 A1019	Q41 S674 K676 K752 N753 N769 I770 Y788 N805 T859 D861 S862 A863

Gene Ontology

	Molecular Function
GO:0003677	DNA binding
GO:0003723	RNA binding
GO:0004519	endonuclease activity
GO:0046872	metal ion binding

	Biological Process
GO:0043571	maintenance of CRISPR repeat elements
GO:0051607	defense response to virus

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Molecular Function

View graph for
Biological Process

External links

PDB	RCSB:8uza, PDBe:8uza, PDBj:8uza
PDBsum	8uza
PubMed	38781968
UniProt	A0A150MP45

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