Structure of PDB 8uo2 Chain A

Receptor sequence

>8uo2A (length=283) Species: 9606 (Homo sapiens) [Search protein sequence]

TEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKSTIVK
QMRILATKVQDIKNNLKEAIETIVAARACYERSNEYQLIDDQDLLRCRIF
ETKFQVDKVNFHMFDVGDERRKWIQCFNDVTAIIFVVASSSYNMVIREDN
QTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDY
FPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYP
HFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL

3D structure

PDB

8uo2 Time-resolved cryo-EM of G protein activation by a GPCR

Chain

Resolution

3.6 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GTP	A	A48 G49 E50 G52 K53 S54 L198 R199 C200 R201 K293 L296 A366	A40 G41 E42 G44 K45 S46 L95 R96 C97 R98 K182 L185 A255

Gene Ontology

	Molecular Function
GO:0003924	GTPase activity
GO:0003925	G protein activity
GO:0005515	protein binding
GO:0005525	GTP binding
GO:0010856	adenylate cyclase activator activity
GO:0016787	hydrolase activity
GO:0019001	guanyl nucleotide binding
GO:0031683	G-protein beta/gamma-subunit complex binding
GO:0031748	D1 dopamine receptor binding
GO:0046872	metal ion binding

	Biological Process
GO:0002862	negative regulation of inflammatory response to antigenic stimulus
GO:0003091	renal water homeostasis
GO:0007165	signal transduction
GO:0007186	G protein-coupled receptor signaling pathway
GO:0007189	adenylate cyclase-activating G protein-coupled receptor signaling pathway
GO:0007190	activation of adenylate cyclase activity
GO:0007191	adenylate cyclase-activating dopamine receptor signaling pathway
GO:0007608	sensory perception of smell
GO:0046907	intracellular transport
GO:0048589	developmental growth
GO:0050796	regulation of insulin secretion
GO:0050890	cognition
GO:0060348	bone development
GO:0060789	hair follicle placode formation
GO:0070527	platelet aggregation
GO:0071377	cellular response to glucagon stimulus
GO:0071380	cellular response to prostaglandin E stimulus
GO:0071870	cellular response to catecholamine stimulus
GO:0071880	adenylate cyclase-activating adrenergic receptor signaling pathway
GO:0120162	positive regulation of cold-induced thermogenesis

	Cellular Component
GO:0005737	cytoplasm
GO:0005829	cytosol
GO:0005834	heterotrimeric G-protein complex
GO:0005886	plasma membrane
GO:0016020	membrane
GO:0032588	trans-Golgi network membrane
GO:0070062	extracellular exosome

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Molecular Function

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Biological Process

View graph for
Cellular Component

External links

PDB	RCSB:8uo2, PDBe:8uo2, PDBj:8uo2
PDBsum	8uo2
PubMed	38480881
UniProt	P63092\|GNAS2_HUMAN Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (Gene Name=GNAS)

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