Structure of PDB 8uk4 Chain A

Receptor sequence

>8uk4A (length=426) Species: 9606 (Homo sapiens)

ATGQDRVVALVDMDCFFVQVEQRQNPHLRNKPCAVVQYKSWKGGGIIAVS
YEARAFGVTRSMWADDAKKLCPDLLLAQVRESRGKANLTKYREASVEVME
IMSRFAVIERASIDEAYVDLTSAVQERLQKLQGQPISADLLPSTYIEGLP
QGPTVQKEGMRKQGLFQWLDSLQIDNLTSPDLQLTVGAVIVEEMRAAIER
ETGFQCSAGISHNKVLAKLACGLNKPNRQTLVSHGSVPQLFSQMPIRKIR
SLGGKLGASVIEILGIEYMGELTQFTESQLQSHFGEKNGSWLYAMCRGIE
HDPVKPRQLPKTIGCSKNFPGKTALATREQVQWWLLQLAQELEERLTKDR
NDNDRVATQLVVSIRVQGDKRLSSLRRCCALTRYDAHKMSHDAFTVIKNC
NTSGIQTEWSPPLTMLFLCATKFSAS

3D structure

• PDB: 8uk4 Replication Bypass of the N -(2-Deoxy-d-erythro-pentofuranosyl)-urea DNA Lesion by Human DNA Polymerase eta.
• Chain: A
• Resolution: 3.02 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	Q38 Y39 W42 S62 W64 K86 A87 K311 P316 K317 T318 G320 C321 S322 K323 N324 P326 T329 R351	Q37 Y38 W41 S61 W63 K85 A86 K305 P310 K311 T312 G314 C315 S316 K317 N318 P320 T323 R345
BS02	dna	A	S113 E116 K224 R256 S257 G259 G260 K261 R377 L381 R382 R383 C384	S112 E115 K218 R250 S251 G253 G254 K255 R371 L375 R376 R377 C378
BS03	1FZ	A	D13 M14 D15 C16 F17 F18 Y52 R55 R61 D115 K231	D12 M13 D14 C15 F16 F17 Y51 R54 R60 D114 K225
BS04	MG	A	D13 M14 D115	D12 M13 D114

Gene Ontology

Molecular Function

• GO:0003684 damaged DNA binding

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:8uk4, PDBe:8uk4, PDBj:8uk4
• PDBsum: 8uk4
• PubMed: 38413007
• UniProt: Q9Y253|POLH_HUMAN DNA polymerase eta (Gene Name=POLH)