Structure of PDB 8uha Chain A

Receptor sequence
>8uhaA (length=1417) Species: 9823 (Sus scrofa) [Search protein sequence]
ACPLRTIKRVQFGVLSPDELKRMSVTEGGIKYPETTEGGRPKLGGLMDPR
QGVIERTGRCQTCAGNMTECPGHFGHIELAKPVFHVGFLVKTMKVLRCVC
FFCSKLLVDSNNPKIKDILAKSKGQPKKRLTHVYDLCKGKNICEGGCGRY
QPRIRRSGLELYAEWKKILLSPERVHEIFKRISDEECFVLGMEPRYARPE
WMIVTVLPVPPLSVRPAVVMQGSARNQDDLTHKLADIVKINNQLRRNEQN
GAAAHVIAEDVKLLQFHVATMVDNELPGLPRAMQKSGRPLKSLKQRLKGK
EGRVRGNLMGKRVDFSARTVITPDPNLSIDQVGVPRSIAANMTFAEIVTP
FNIDRLQELVRRGNSQYPGAKYIIRDNGDRIDLRFHPKPSDLHLQTGYKV
ERHMCDGDIVIFNRQPTLHKMSMMGHRVRILPWSTFRLNLSVTTPYNADF
DGDEMNLHLPQSLETRAEIQELAMVPRMIVTPQSNRPVMGIVQDTLTAVR
KFTKRDVFLERGEVMNLLMFLSTWDGKVPQPAILKPRPLWTGKQIFSLII
PGHINCIRTHSTHPDDEDSGPYKHISPGDTKVVVENGELIMGILCKKSLG
TSAGSLVHISYLEMGHDITRLFYSNIQTVINNWLLIEGHTIGIGDSIADS
KTYQDIQNTIKKAKQDVIEVIEKAHNNELEPTPGNTLRQTFENQVNRILN
DARDKTGSSAQKSLSEYNNFKSMVVSGAKGSKINISQVIAVVGQQNVEGK
RIPFGFKHRTLPHFIKDDYGPESRGFVENSYLAGLTPTEFFFHAMGGREG
LIDTAVKTAETGYIQRRLIKSMESVMVKYDATVRNSINQVVQLRYGEDGL
AGESVEFQNLATLKPSNKAFEKKFRFDYTNERALRRTLQEDLVKDVLSNA
HIQNELEREFERMREDREVLRVIFPTGDSKVVLPCNLLRMIWNAQKIFHI
NPRLPSDLHPIKVVEGVKELSKKLVIVNGDDPLSRQAQENATLLFNIHLR
STLCSRRMAEEFRLSGEAFDWLLGEIESKFNQAIAHPGEMVGALAAQSLG
EPATQMTLNKNVTLGVPRLKELINISKKPKTPSLTVFLLGQSARDAERAK
DILCRLEHTTLRKVTANTAIYYDPNPQSTVVAEDQEWVNVYYEMPDFDVA
RISPWLLRVELDRKHMTDRKLTMEQIAEKINAGFGDDLNCIFNDDNAEKL
VLRIRIMNSDDDDVFLRCIESNMLTDMTLQGIEQISKVYMHLPQTDNKKK
IIITEDGEFKALQEWILETDGVSLMRVLSEKDVDPVRTTSNDIVEIFTVL
GIEAVRKALERELYHVISFDGSYVNYRHLALLCDTMTCRGHLMAITRHGV
NRQDTGPLMKCSFEETVDVLMEAAAHGESDPMKGVSENIMLGQLAPAGTG
CFDLLLDAEKCKYGMEI
3D structure
PDB8uha Distinct negative elongation factor conformations regulate RNA polymerase II promoter-proximal pausing.
ChainA
Resolution3.5 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 2.7.7.6: DNA-directed RNA polymerase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 rna A R67 V264 V265 M266 R334 K337 F431 D497 D499 R56 V218 V219 M220 R288 K291 F385 D451 D453
BS02 dna A K331 K1132 H1417 K285 K1077 H1348
BS03 dna A M266 K346 R364 Q461 T854 R862 E1433 E1434 M220 K300 R318 Q415 T808 R816 E1364 E1365
BS04 MG A D495 D497 D449 D451
BS05 ZN A C71 T73 C81 H84 C60 T62 C70 H73
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003899 DNA-directed 5'-3' RNA polymerase activity
GO:0016779 nucleotidyltransferase activity
GO:0046872 metal ion binding
Biological Process
GO:0006351 DNA-templated transcription
GO:0006366 transcription by RNA polymerase II
Cellular Component
GO:0000428 DNA-directed RNA polymerase complex
GO:0005634 nucleus
GO:0031981 nuclear lumen
GO:0032991 protein-containing complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:8uha, PDBe:8uha, PDBj:8uha
PDBsum8uha
PubMed38401543
UniProtA0A8D1DPV6

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