Structure of PDB 8u85 Chain A

Receptor sequence

>8u85A (length=619) Species: 9606 (Homo sapiens)

EEDARWLRWVTQQFKTIISLQEFKAALHVESFFAERFFALFDTLQELQEA
LTLLIHSPMDKLKFLFQVYDIDPDELRTVLQSCLRESAISLPDEKLDQLT
LALFESADNGAITFEELRDELQRFPGVMENLTISAAQLTRAYWHNHRSQL
FCLATYAGLHVLLFGLAASAHRDLGASVMVAKGCGQCLNFDCSFIAVLML
RRCLTWLRATWLAQVLPLDQNIQFHQLMGYVVVGLSLVHTVAHTVNFVLQ
AQHGSASPTGVALLLLLLLMFICSSSCIRRSGHFEVFYWTHLSYLLVWLL
LIFHGPNFWKWLLVPGILFFLEKAIGLAVSRMAAVCIMEVNLLPSKVTHL
LIKRPPFFHYRPGDYLYLNIPTIARYEWHPFTISSAPEQKDTIWLHIRSQ
GQWTNRLYESFKASCNIKCYIDGPYGTPTRRIFASEHAVLIGAGIGITPF
ASILQSIMYRHQKRKHTCPSCQHSWIEGVQDNMKLHKVDFIWINRDQRSF
EWFVSLLTKLEMDQAEEAQYGRFLELHMYMTSALGKNDMKAIGLQMALDL
LANKEKKDSITGLQTRTQPGRPDWSKVFQKVAAEKKGKVQVFFCGSPALA
KVLKGHCEKFGFRFFQENF

3D structure

• PDB: 8u85 Structural Basis of Human NOX5 Activation
• Chain: A
• Resolution: 3.2 Å

Enzymatic activity

• Enzyme Commision number: 1.6.3.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	HEB	A	N232 S279 H282 T283 H286 G325 V326 L328 L329 L366 H369 P371	N189 S236 H239 T240 H243 G260 V261 L263 L264 L301 H304 P306
BS02	HEB	A	I238 M242 H268 G272 M335 F336 S339 F352 H356 Y359	I195 M199 H225 G229 M270 F271 S274 F287 H291 Y294
BS03	NDP	A	H189 A543 N594 R595 T631 R671 P672 W674 S696 L699	H146 A443 N494 R495 T531 R571 P572 W574 S596 L599
BS04	FAD	A	R344 Y430 H444 P445 F446 T447 H461 R463 G466 Q467 W468 T469	R279 Y365 H379 P380 F381 T382 H396 R398 G401 Q402 W403 T404
BS05	ZN	A	C568 C571	C468 C471

Gene Ontology

Molecular Function

• GO:0005509 calcium ion binding
• GO:0015252 proton channel activity
• GO:0016175 superoxide-generating NAD(P)H oxidase activity
• GO:0016491 oxidoreductase activity
• GO:0020037 heme binding
• GO:0046872 metal ion binding
• GO:0050660 flavin adenine dinucleotide binding
• GO:0050661 NADP binding
• GO:0050664 oxidoreductase activity, acting on NAD(P)H, oxygen as acceptor
• GO:0106292 superoxide-generating NADPH oxidase activity

Biological Process

• GO:0001525 angiogenesis
• GO:0001819 positive regulation of cytokine production
• GO:0001935 endothelial cell proliferation
• GO:0006915 apoptotic process
• GO:0006952 defense response
• GO:0034220 monoatomic ion transmembrane transport
• GO:0042554 superoxide anion generation
• GO:0043012 regulation of fusion of sperm to egg plasma membrane
• GO:0061640 cytoskeleton-dependent cytokinesis
• GO:1902600 proton transmembrane transport

Cellular Component

• GO:0005783 endoplasmic reticulum
• GO:0005789 endoplasmic reticulum membrane
• GO:0005886 plasma membrane
• GO:0043020 NADPH oxidase complex

External links

• PDB: RCSB:8u85, PDBe:8u85, PDBj:8u85
• PDBsum: 8u85
• UniProt: Q96PH1|NOX5_HUMAN NADPH oxidase 5 (Gene Name=NOX5)