Structure of PDB 8u3y Chain A

Receptor sequence
>8u3yA (length=1336) Species: 1314 (Streptococcus pyogenes) [Search protein sequence]
KKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALL
FDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLR
LIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPIN
ASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNF
KSNFDLAEDAKLQLSKDTYDDDLDNLLAQIDLFLAAKNLSDAILLSDILR
VNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKN
GYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDN
GSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLAR
GNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKV
LPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNR
KVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFL
DNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYT
GWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKED
IQKAQDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIE
MARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDK
NRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELD
KAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKL
VSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGD
YATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDF
ATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKK
YGGFLWPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPID
FLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAKQLQKGNELALPS
KYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRV
ILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTT
IDRKQYRSTKEVLDATLIHQSITGLYETRIDLSQLG
3D structure
PDB8u3y Unraveling the mechanisms of PAMless DNA interrogation by SpRY-Cas9
ChainA
Resolution3.1 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.-.-
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 rna A S29 K33 L35 K44 N46 E57 T58 T62 R63 K65 R66 R69 R70 R71 Y72 R74 R75 K76 N77 L101 S104 F105 K111 R115 H116 I122 E125 H129 T134 I135 H160 K163 F164 R165 G166 Y325 H328 H329 K336 R340 F351 F352 Y359 A360 I363 D364 Q402 R403 T404 F405 N407 G408 F446 R447 I448 Y450 G453 P454 L455 A456 R457 N459 S460 F462 W464 T472 I473 F491 K510 Y515 T519 N588 R661 M694 H721 P731 K735 R765 S777 R778 R780 N803 K848 D850 S851 K918 R919 Y981 G1011 Y1013 P1090 Q1091 G1103 G1104 F1105 I1110 P1112 R1122 K1123 K1124 W1126 K1130 Y1131 M1169 E1225 A1227 Q1272 H1349 Q1350 S1351 Y1356 S27 K31 L33 K42 N44 E55 T56 T60 R61 K63 R64 R67 R68 R69 Y70 R72 R73 K74 N75 L99 S102 F103 K109 R113 H114 I120 E123 H127 T132 I133 H158 K161 F162 R163 G164 Y318 H321 H322 K329 R333 F344 F345 Y352 A353 I356 D357 Q395 R396 T397 F398 N400 G401 F439 R440 I441 Y443 G446 P447 L448 A449 R450 N452 S453 F455 W457 T465 I466 F484 K503 Y508 T512 N581 R654 M687 H709 P719 K723 R753 S765 R766 R768 N791 K836 D838 S839 K906 R907 Y969 G999 Y1001 P1060 Q1061 G1073 G1074 F1075 I1080 P1082 R1092 K1093 K1094 W1096 K1100 Y1101 M1139 E1195 A1197 Q1242 H1319 Q1320 S1321 Y1326
BS02 dna A S845 N863 D868 K1107 E1108 S1109 S833 N851 D856 K1077 E1078 S1079
BS03 dna A Y450 M495 N497 G582 E584 N588 R655 W659 R661 M694 H698 R765 E766 Q768 T770 G773 Q774 K810 D837 V838 H840 Q844 Q926 K929 Y1013 A1032 Y443 M488 N490 G575 E577 N581 R648 W652 R654 M687 H691 R753 E754 Q756 T758 G761 Q762 K798 D825 V826 H828 Q832 Q914 K917 Y1001 A1002
BS04 dna A L1135 W1136 K1200 S1216 Q1219 Q1221 R1333 Q1335 R1337 L1105 W1106 K1170 S1186 Q1189 Q1191 R1303 Q1305 R1307
BS05 MG A D10 E762 D8 E750
BS06 MG A D10 H983 D8 H971
BS07 MG A D839 N863 D827 N851
Gene Ontology
Molecular Function
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0004519 endonuclease activity
GO:0004520 DNA endonuclease activity
GO:0004527 exonuclease activity
GO:0008408 3'-5' exonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0043571 maintenance of CRISPR repeat elements
GO:0051607 defense response to virus

View graph for
Molecular Function
View graph for
Biological Process
External links
PDB RCSB:8u3y, PDBe:8u3y, PDBj:8u3y
PDBsum8u3y
PubMed38688943
UniProtQ99ZW2|CAS9_STRP1 CRISPR-associated endonuclease Cas9/Csn1 (Gene Name=cas9)

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