Structure of PDB 8tzz Chain A

Receptor sequence

>8tzzA (length=1336) Species: 1314 (Streptococcus pyogenes)

KKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALL
FDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLR
LIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPIN
ASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNF
KSNFDLAEDAKLQLSKDTYDDDLDNLLAQIDLFLAAKNLSDAILLSDILR
VNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKN
GYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDN
GSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLAR
GNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKV
LPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNR
KVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFL
DNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYT
GWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKED
IQKAQDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIE
MARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDK
NRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELD
KAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKL
VSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGD
YATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDF
ATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKK
YGGFLWPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPID
FLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAKQLQKGNELALPS
KYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRV
ILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTT
IDRKQYRSTKEVLDATLIHQSITGLYETRIDLSQLG

3D structure

• PDB: 8tzz Unraveling the mechanisms of PAMless DNA interrogation by SpRY-Cas9
• Chain: A
• Resolution: 3.56 Å

Enzymatic activity

• Enzyme Commision number: 3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	K30 L35 I43 K44 N46 E57 T58 A59 T62 R63 K65 R66 R69 R70 R71 Y72 R74 R75 K76 L101 S104 F105 E108 R115 H116 I122 E125 H129 I135 H160 K163 F164 G166 H167 Y325 H328 H329 K336 R340 F351 F352 I363 Q402 R403 T404 F405 N407 F446 R447 I448 Y450 G453 L455 A456 N459 S460 F462 W464 I473 F491 Y515 N588 R661 M694 S719 H721 S730 P731 K735 L738 K742 K749 R753 R765 S777 R778 R780 N803 K848 D850 S851 Y981 Y1010 Y1013 M1089 P1090 Q1091 E1099 V1100 T1102 G1103 G1104 F1105 E1108 I1110 P1112 K1113 R1122 K1124 Y1131 F1134 M1169 E1225 A1227 Q1272 H1349 Q1350 S1351 Y1356	K28 L33 I41 K42 N44 E55 T56 A57 T60 R61 K63 R64 R67 R68 R69 Y70 R72 R73 K74 L99 S102 F103 E106 R113 H114 I120 E123 H127 I133 H158 K161 F162 G164 H165 Y318 H321 H322 K329 R333 F344 F345 I356 Q395 R396 T397 F398 N400 F439 R440 I441 Y443 G446 L448 A449 N452 S453 F455 W457 I466 F484 Y508 N581 R654 M687 S707 H709 S718 P719 K723 L726 K730 K737 R741 R753 S765 R766 R768 N791 K836 D838 S839 Y969 Y998 Y1001 M1059 P1060 Q1061 E1069 V1070 T1072 G1073 G1074 F1075 E1078 I1080 P1082 K1083 R1092 K1094 Y1101 F1104 M1139 E1195 A1197 Q1242 H1319 Q1320 S1321 Y1326
BS02	dna	A	S845 N863 R864 K1107 E1108 S1109 R1337	S833 N851 R852 K1077 E1078 S1079 R1307
BS03	dna	A	L169 Y450 N497 K526 G582 E584 N588 R655 T657 W659 R765 E766 T770 G773 K810 D837 V838 D839 H840 N863 R864 R925 Q926 K929 A1032	L167 Y443 N490 K519 G575 E577 N581 R648 T650 W652 R753 E754 T758 G761 K798 D825 V826 D827 H828 N851 R852 R913 Q914 K917 A1002
BS04	dna	A	R1114 K1118 L1135 W1136 K1200 S1216 Q1219 Q1221 A1320 R1333 Q1335	R1084 K1088 L1105 W1106 K1170 S1186 Q1189 Q1191 A1290 R1303 Q1305

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003723 RNA binding
• GO:0004519 endonuclease activity
• GO:0004520 DNA endonuclease activity
• GO:0004527 exonuclease activity
• GO:0008408 3'-5' exonuclease activity
• GO:0046872 metal ion binding

Biological Process

• GO:0043571 maintenance of CRISPR repeat elements
• GO:0051607 defense response to virus

External links

• PDB: RCSB:8tzz, PDBe:8tzz, PDBj:8tzz
• PDBsum: 8tzz
• PubMed: 38688943
• UniProt: Q99ZW2|CAS9_STRP1 CRISPR-associated endonuclease Cas9/Csn1 (Gene Name=cas9)