Structure of PDB 8tol Chain A

Receptor sequence

>8tolA (length=670) Species: 660122 (Fusarium vanettenii 77-13-4)

ESNFGVDFVIHYKVPAAERDEAEAGFVQLIRALTTVGLATEVRHGENESL
LVFVKVASPDLFAKQVYRARLGDWLHGVRVSAPHNDIAQALQDEPVVEAE
RLRLIYLMITKPHNEGGAGVTPTNAKWKHVESIFPLHSHSFNKEWIKKWS
SKYTLEQTDIDNIRDKFGESVAFYFAFLRSYFRFLVIPSAFGFGAWLLLG
QFSYLYALLCGLWSVVFFEYWKKQEVDLAVQWGVRGVSSIQQSRPEFEWE
HEAEDPITGEPVKVYPPMKRVKTQLLQIPFALACVVALGALIVTCNSLEV
FINEVYSGPGKQYLGFLPTIFLVIGTPTISGVLMGAAEKLNAMENYATVD
AHDAALIQKQFVLNFMTSYMALFFTAFVYIPFGHILHPFLNFWRATAQTL
TFSEKELPTREFQINPARISNQMFYFTVTAQIVNFATEVVVPYIKQQAFQ
KAKQLKEDHEEEAEFLQRVREECTLEEYDVSGDYREMVMQFGYVAMFSVA
WPLAACCFLVNNWVELRSDALKIAISSRRPIPWRTDSIGPWLTALSFLSW
LGSITSSAIVYLCSNSSPLKAWGLLLSILFAEHFYLVVQLAVRFVLSKLD
SPGLQKERKERFQTKKRLLQENSEKITREALEEEARGHGTPEEMFWQRQR
GMQETIEIGRRMIEQQLAAG

3D structure

• PDB: 8tol Structural basis of closed groove scrambling by a TMEM16 protein.
• Chain: A
• Resolution: 2.85 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CA	A	E452 D503 E506 E535 D539	E438 D483 E486 E515 D519
BS02	CA	A	N448 E452 E506 E535	N434 E438 E486 E515
BS03	PGW	A	F396 L597 L602	F382 L569 L574
BS04	PGW	A	I201 Y234	I187 Y220
BS05	PGW	A	V615 L618	V587 L590

Gene Ontology

Molecular Function

• GO:0005254 chloride channel activity
• GO:0042802 identical protein binding
• GO:0046872 metal ion binding

Biological Process

• GO:1902476 chloride transmembrane transport

Cellular Component

• GO:0016020 membrane
• GO:0032541 cortical endoplasmic reticulum

External links

• PDB: RCSB:8tol, PDBe:8tol, PDBj:8tol
• PDBsum: 8tol
• PubMed: 38684930
• UniProt: C7Z7K1