Structure of PDB 8t7s Chain A

Receptor sequence
>8t7sA (length=1312) Species: 1314 (Streptococcus pyogenes) [Search protein sequence]
KKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALL
FDSGETAERTRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLR
LIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLAKAILSA
RLSKSRRLENLIAQKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKD
TYDDDLDNLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDL
TLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEK
MDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPF
LKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVV
DKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTE
GMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISG
VEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMI
EERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDF
LKSDGFANRNFMQLIHDDSLTFKEDIQKAQDSLHEHIANLAGSPAIKKGI
LQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEG
IKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYD
VDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLL
NAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDS
RMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDA
YLNAVVGTALIKKYPKLESEFVYGIGKATAKYFFYSNIMNFFKTEITLAN
GEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGG
FSKESIRPKRNSDKLIARKKDWDPKKYGGFLWPTVAYSVLVVAKVEKGKS
KKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLF
ELENGRKRMLASAKQLQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQ
KQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIRE
QAENIIHLFTLTRLGAPRAFKYFDTTIDPKQYRSTKEVLDATLIHQSITG
LYETRIDLSQLG
3D structure
PDB8t7s Unraveling the mechanisms of PAMless DNA interrogation by SpRY-Cas9
ChainA
Resolution3.01 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.-.-
Interaction with ligand
Site
#
Ligand Ligand
chain
Binding residues on receptor
(original residue number in PDB)
Binding residues on receptor
(residue number reindexed from 1)
Binding affinity
BS01 rna A K30 K33 R40 I43 K44 N46 G56 E57 T58 A59 R61 T62 R63 K65 R66 R69 R70 R71 Y72 R74 R75 K76 N77 L101 S104 F105 L106 E108 K111 R115 H116 P117 I122 V126 H129 H160 K163 F164 R165 G166 Y325 H328 K336 R340 F351 F352 Y359 A360 I363 Q402 R403 T404 F405 N407 G408 F446 R447 I448 Y450 G453 P454 L455 A456 R457 N459 S460 F462 W464 R467 T472 I473 Y515 F518 T519 N588 R661 L662 M694 S719 H721 E722 S730 P731 K735 L738 K742 K749 R753 S777 R778 E779 R780 N803 Y812 K848 D849 D850 S851 I852 K918 R919 Y981 S1006 Y1010 P1090 Q1091 E1099 V1100 T1102 G1103 G1104 F1105 I1110 P1112 K1113 R1122 K1123 K1124 K1130 Y1131 F1134 E1225 L1226 A1227 P1229 Q1272 H1349 Q1350 S1351 Y1356 K28 K31 R38 I41 K42 N44 G54 E55 T56 A57 R59 T60 R61 K63 R64 R67 R68 R69 Y70 R72 R73 K74 N75 L99 S102 F103 L104 E106 K109 R113 H114 P115 I120 V124 H127 H158 K161 F162 R163 G164 Y293 H296 K304 R308 F319 F320 Y327 A328 I331 Q370 R371 T372 F373 N375 G376 F414 R415 I416 Y418 G421 P422 L423 A424 R425 N427 S428 F430 W432 R435 T440 I441 Y483 F486 T487 N556 R629 L630 M662 S682 H684 E685 S693 P694 K698 L701 K705 K712 R716 S740 R741 E742 R743 N766 Y775 K811 D812 D813 S814 I815 K881 R882 Y944 S969 Y973 P1036 Q1037 E1045 V1046 T1048 G1049 G1050 F1051 I1056 P1058 K1059 R1068 K1069 K1070 K1076 Y1077 F1080 E1171 L1172 A1173 P1175 Q1218 H1295 Q1296 S1297 Y1302
BS02 dna A S845 N863 R864 S867 D868 K1107 E1108 S1109 R1111 W1136 S808 N826 R827 S830 D831 K1053 E1054 S1055 R1057 W1082
BS03 dna A L169 Y450 I492 M495 T496 N497 K526 G582 N588 R655 T657 W659 R661 R765 E766 T770 G773 Q774 K810 S834 D837 V838 D839 H840 N863 R925 Q926 K929 K1031 A1032 L167 Y418 I460 M463 T464 N465 K494 G550 N556 R623 T625 W627 R629 R728 E729 T733 G736 Q737 K773 S797 D800 V801 D802 H803 N826 R888 Q889 K892 K977 A978
BS04 dna A L1135 W1136 P1137 K1153 K1200 S1216 K1218 Q1219 Q1221 A1320 R1337 L1081 W1082 P1083 K1099 K1146 S1162 K1164 Q1165 Q1167 A1266 R1283
BS05 MG A D10 E762 D8 E725
BS06 MG A D10 H983 D8 H946
BS07 MG A D839 N863 D802 N826
Gene Ontology
Molecular Function
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0004519 endonuclease activity
GO:0004520 DNA endonuclease activity
GO:0004527 exonuclease activity
GO:0008408 3'-5' exonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0043571 maintenance of CRISPR repeat elements
GO:0051607 defense response to virus

View graph for
Molecular Function

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Biological Process
External links
PDB RCSB:8t7s, PDBe:8t7s, PDBj:8t7s
PDBsum8t7s
PubMed38688943
UniProtQ99ZW2|CAS9_STRP1 CRISPR-associated endonuclease Cas9/Csn1 (Gene Name=cas9)

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