Structure of PDB 8t6p Chain A

Receptor sequence

>8t6pA (length=1108) Species: 1314 (Streptococcus pyogenes)

KKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALL
FDSGETAERTRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLR
LIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPIN
ASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNF
KSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILL
SDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFF
DQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQ
RTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYV
GPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNL
PNEKVLPKHSLLYEYFTVYNELTKGEQKKAIVDLLFKTNRKVTVKQLKED
YFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILE
DIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLI
NGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSSLH
EHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMQITKHVAQ
ILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHH
AHDAYLNAVVGTALIKKKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SIRPKRNSDKLIARKKDWDPKKYGGFLWPTVAYSVLVVAKVEKGKSKKLK
SVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELEN
GRKRMLASAKQLQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLF
VEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAEN
IIHLFTLTRLGAPRAFKYFDTTIDPKQYRSTKEVLDATLIHQSITGLYET
RIDLSQLG

3D structure

• PDB: 8t6p Unraveling the mechanisms of PAMless DNA interrogation by SpRY-Cas9
• Chain: A
• Resolution: 3.15 Å

Enzymatic activity

• Enzyme Commision number: 3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	K30 K33 R40 I43 K44 N46 G56 E57 T58 A59 R61 T62 R63 R66 R69 R70 R71 Y72 R74 R75 N77 R78 Y81 L101 S104 F105 R115 H116 I122 E125 V126 H129 I135 H160 K163 F164 R165 G166 D269 Y271 Y325 H328 H329 K336 F351 F352 Y359 A360 I363 Q402 R403 T404 F405 N407 G408 F446 R447 I448 Y450 G453 P454 L455 A456 R457 N459 S460 F462 W464 R467 T472 I473 P475 M495 N497 S730 Q739 K742 R753 P1090 Q1091 K1097 V1100 G1103 G1104 F1105 I1110 P1112 K1113 R1122 K1123 K1124 K1130 Y1131 E1225 A1227 Q1272 H1349 Q1350 S1351 Y1356 T1358	K28 K31 R38 I41 K42 N44 G54 E55 T56 A57 R59 T60 R61 R64 R67 R68 R69 Y70 R72 R73 N75 R76 Y79 L99 S102 F103 R113 H114 I120 E123 V124 H127 I133 H158 K161 F162 R163 G164 D267 Y269 Y323 H326 H327 K334 F349 F350 Y357 A358 I361 Q400 R401 T402 F403 N405 G406 F444 R445 I446 Y448 G451 P452 L453 A454 R455 N457 S458 F460 W462 R465 T470 I471 P473 M493 N495 S709 Q718 K721 R732 P832 Q833 K839 V842 G845 G846 F847 I852 P854 K855 R864 K865 K866 K872 Y873 E967 A969 Q1014 H1091 Q1092 S1093 Y1098 T1100
BS02	dna	A	D269 K1107 E1108 S1109 R1111 W1136	D267 K849 E850 S851 R853 W878
BS03	dna	A	R1114 K1118 L1135 W1136 S1216 Q1219 Q1221 A1320 R1322	R856 K860 L877 W878 S958 Q961 Q963 A1062 R1064

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003723 RNA binding
• GO:0004519 endonuclease activity
• GO:0004520 DNA endonuclease activity
• GO:0004527 exonuclease activity
• GO:0008408 3'-5' exonuclease activity
• GO:0046872 metal ion binding

Biological Process

• GO:0043571 maintenance of CRISPR repeat elements
• GO:0051607 defense response to virus

External links

• PDB: RCSB:8t6p, PDBe:8t6p, PDBj:8t6p
• PDBsum: 8t6p
• PubMed: 38688943
• UniProt: Q99ZW2|CAS9_STRP1 CRISPR-associated endonuclease Cas9/Csn1 (Gene Name=cas9)