Structure of PDB 8t6l Chain A

Receptor sequence

>8t6lA (length=1237) Species: 6100,10116

SSFRRFTRESLAAIEKRMAEKQPRPQLDLQASKKLPDLYGNPPRELIGEP
LEDLDPFYSTQKTFIVLNKGKTIFRFSATNALYVLSPFHPVRRAAVKILV
HSLFSMLIMCTILTNCVFMAQHDPPPWTKYVEYTFTAIYTFESLVKILAR
GFCLHAFTFLRDPWNWLDFSVIVMAYTTEFVSALRTFRVLRALKTISVIS
GLKTIVGALIQSVKKLADVMVLTVFCLSVFALIGLQLFMGNLRHKCVRNF
TELNGTNGSVEADGLVWNSLDVYLNDPANYLLKNGTTDVLLCGNSSDAGT
CPEGYRCLKAGENPDHGYTSFDSFAWAFLALFRLMTQDCWERLYQQTLRS
AGKIYMIFFMLVIFLGSFYLVNLILAVVAMAYEEQNQATIAETEEKEKRF
CCPLWMSIKQKVKFVVMDPFADLTITMCIVLNTLFMALEHYNMTAEFEEM
LQVGNLVFTGIFTAEMTFKIIALDPYYYFQQGWNIFDSIIVILSLMELGL
SRMGNLSVLRSFRLLRVFKLAKSWPTLNTLIKIIGNSVGALGNLTLVLAI
IVFIFAVVGMQLFGKNYSELRHRISDSGLLPRWHMMDFFHAFLIIFRILC
GEWIETMWDCMEVSGQSLCLLVFLLVMVIGNLVVLNLFLALLLSSFGKVW
WRLRKTCYRIVEHSWFETFIIFMILLSSGALAFEDIYLEERKTIKVLLEY
ADKMFTYVFVLEMLLKWVAYGFKKYFTNAWCWLDFLIVDVSLVSLVANTL
GFAEMGPIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVC
LIFWLIFSIMGVNLFAGKFGRCINQTEGDLPLNYTIVNNKSECESFNVTG
ELYWTKVKVNFDNVGAGYLALLQVATFKGWMDIMYAAVDSRGYEEQPQWE
DNLYMYIYFVVFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQDIFMTEEQ
KKYYNAMKKLGSKKPQKPIPRPLNKYQGFIFDIVTKQAFDVTIMFLICLN
MVTMMVETDDQSPEKVNILAKINLLFVAIFTGECIVKMAALRHYYFTNSW
NIFDFVVVILSIVGTVLSDIIQKYFFSPTLFRVIRLARIGRILRLIRGAK
GIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMANFAYVKWEAGIDDM
FNFQTFANSMLCLFQITTSAGWDGLLSPILNTGPPYCDPNLPNSNGSRGN
CGSPAVGILFFTTYIIISFLIVVNMYIAIILENFSVA

3D structure

• PDB: 8t6l Dual receptor-sites reveal the structural basis for hyperactivation of sodium channels by poison-dart toxin batrachotoxin.
• Chain: A
• Resolution: 3.3 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	LBN	A	F363 Q915 S916 L919 L924	F328 Q616 S617 L620 L625
BS02	LBN	A	G781 F811	G482 F512
BS03	LBN	A	N842 L845	N543 L546
BS04	LBN	A	M136 M139	M106 M109
BS05	LBN	A	S1303 T1306 L1665 Y1673 F1676 N1680 V1749 F1753	S760 T763 L1122 Y1130 F1133 N1137 V1206 F1210
BS06	LBN	A	M1337 L1340 C1343 G1408 A1748 L1752 T1755 I1759	M794 L797 C800 G865 A1205 L1209 T1212 I1216
BS07	LBN	A	A1272 W1273	A729 W730
BS08	Y01	A	A218 F222 F854	A183 F187 F555
BS09	Y01	A	P193 W194	P163 W164
BS10	Y01	A	L1344 N1406	L801 N863
BS11	Y01	A	L737 N1406	L438 N863
BS12	Y01	A	V729 L819 I1345	V430 L520 I802
BS13	Y01	A	Y390 F393	Y355 F358
BS14	Y01	A	K388 I389 M395 M1703	K353 I354 M360 M1160
BS15	Y01	A	T1546 V1549 E1557	T1003 V1006 E1014
BS16	9Z9	A	F811 M1353 L1357 V1454	F512 M810 L814 V911
BS17	9Z9	A	F887 F888 Y1447 I1450	F588 F589 Y904 I907
BS18	YIJ	A	Q372 I398 F399 G1663 L1666 F1707 T1711 V1765 M1768	Q337 I363 F364 G1120 L1123 F1164 T1168 V1222 M1225
BS19	YIJ	A	C899 W1347 L1415 A1418 T1419 F1420 T1463 L1464 S1712 F1762	C600 W804 L872 A875 T876 F877 T920 L921 S1169 F1219

Gene Ontology

Molecular Function

• GO:0003674 molecular_function
• GO:0005216 monoatomic ion channel activity
• GO:0005248 voltage-gated sodium channel activity
• GO:0005261 monoatomic cation channel activity

Biological Process

• GO:0006091 generation of precursor metabolites and energy
• GO:0006811 monoatomic ion transport
• GO:0006814 sodium ion transport
• GO:0008218 bioluminescence
• GO:0055085 transmembrane transport

Cellular Component

• GO:0001518 voltage-gated sodium channel complex
• GO:0005575 cellular_component
• GO:0005886 plasma membrane
• GO:0016020 membrane

External links

• PDB: RCSB:8t6l, PDBe:8t6l, PDBj:8t6l
• PDBsum: 8t6l
• PubMed: 38485923
• UniProt: P15389;
  P42212|GFP_AEQVI Green fluorescent protein (Gene Name=GFP)