Structure of PDB 8t27 Chain A

Receptor sequence

>8t27A (length=504) Species: 837 (Porphyromonas gingivalis)

GSHHHHHHGSMQEVTMWGDSHGVAPNQVRRTLVKVALSESLPPGAKQIRI
GFSLPKETEEKVTALYLLVSDSLAVRDLPDYKGRVSYDSFPISKEDRTTA
LSADSVAGRRFFYLAADIGPVASFSRSDTLTARVEEVAVDGRPLPLKELS
PASRRLYRGYEALFVPGDGGSRNYRIPAILKTANGTLIAMADRRKYNQTA
LPEDIDIVMRRSTDGGKSWSDPRIIVQGEGRNHGFGDVALVQTQAGKLLM
IFVGGVGLWQSTPDRPQRTYISESRDEGLTWSPPRDITHFIFGKDCADPG
RSRWLASFCASGQGLVLPSGRITFVAAIRESGQEYVLNNYVLYSDDEGDT
WQLSDCAYRRGDEAKLSLMPDGRVLMSIRNQGRQESRQRFFALSSDDGLT
WERAKQFEGIHDPGCNGAMLQVKRNGRDQVLHSLPLGPDGRRDGAVYLFD
HVSGRWSAPVVVNSGSSAYSDMTLLADGTIGYFVEEGDEISLVFIRFVLD
DLFD

3D structure

• PDB: 8t27 Functional and structural analyses reveal that a dual domain sialidase protects bacteria from complement killing through desialylation of complement factors.
• Chain: A
• Resolution: 1.41 Å

Enzymatic activity

• Enzyme Commision number: 3.2.1.18: exo-alpha-sialidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SIA	A	R194 I195 D256 F327 D381 E382 R398 R460 Y488	R175 I176 D237 F308 D362 E363 R379 R441 Y469
BS02	GAL	A	Q66 D159 G160	Q47 D140 G141
BS03	GAL	A	G265 K266 R294	G246 K247 R275
BS04	GAL	A	R461 G484 S485 G506 D507	R442 G465 S466 G487 D488

Gene Ontology

Molecular Function

• GO:0004308 exo-alpha-sialidase activity

Biological Process

• GO:0006689 ganglioside catabolic process
• GO:0009313 oligosaccharide catabolic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0016020 membrane
• GO:0043231 intracellular membrane-bounded organelle

External links

• PDB: RCSB:8t27, PDBe:8t27, PDBj:8t27
• PDBsum: 8t27
• PubMed: 37747935
• UniProt: Q7MX62