Structure of PDB 8t1u Chain A

Receptor sequence

>8t1uA (length=353) Species: 3702 (Arabidopsis thaliana)

EPIRLPNPMIGFGVPNEPGLITHRSLPELARGPPFFYYENVALTPKGVWE
TISRHLFEIPPEFVDSKYFCVAARKRGYIHNLPINNRFQIQPPPKYTIHD
AFPLSKRWWPEWDKRTKLNCILTCTGSAQLTNRIRVALEPYNEEPEPPKH
VQRYVIDQCKKWNLVWVGKNKAAPLEPDEMESILGFPKNHTRGGGMSRTE
RFKSLGNSFQVDTVAYHLSVLKPIFPHGINVLSLFTGIGGGEVALHRLQI
KMKLVVSVEISKVNRNILKDFWEQTNQTGELIEFSDIQHLTNDTIEGLME
KYGGFDLVIGGSPCNNLAGGNRVSRVGLEGDQSSLFFEYCRILEVVRARM
RGS

3D structure

• PDB: 8t1u DNA conformational dynamics in the context-dependent non-CG CHH methylation by plant methyltransferase DRM2.
• Chain: A
• Resolution: 2.91 Å

Enzymatic activity

• Enzyme Commision number: 2.1.1.37: DNA (cytosine-5-)-methyltransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	L278 P318 K319 S400 A401 Q402 S470 T472 G592 N594 R595 R598	L5 P45 K46 S127 A128 Q129 S197 T199 G319 N321 R322 R325
BS02	dna	A	E312 V314 R347 K348 R349 N392 C393 L395 T396 C397 G479 N480 C587 N588 G592 G593 R595 V596	E39 V41 R74 K75 R76 N119 C120 L122 T123 C124 G206 N207 C314 N315 G319 G320 R322 V323
BS03	SAH	A	N480 S481 F482 F508 T509 G510 I511 G513 E532 I533 S558 D559 I560 L608	N207 S208 F209 F235 T236 G237 I238 G240 E259 I260 S285 D286 I287 L335

Gene Ontology

Molecular Function

• GO:0008168 methyltransferase activity

External links

• PDB: RCSB:8t1u, PDBe:8t1u, PDBj:8t1u
• PDBsum: 8t1u
• PubMed: 37926286
• UniProt: Q9M548|DRM2_ARATH DNA (cytosine-5)-methyltransferase DRM2 (Gene Name=DRM2)