Structure of PDB 8sts Chain A

Receptor sequence

>8stsA (length=533) Species: 11678 (Human immunodeficiency virus type 1 BH10)

PISPIETVPVKLKPGMDGPKVQWPLTEEKIKALVEICTEMEKEGKISKIG
PYNTPVFAIKLVDFRELNKRTQDFWEVQLGIPHPAGLKKKKSATVLDVGD
AYFSVPLDEDFRKYTAFPSINGIRYQYNVLPQGWKGSPAIFQSSMTKILE
PFAAQNPDIVICQYMDDLYVGSDLEIGQHRTKIEELRQHLLRWGLTTPDK
KHQKEPPFLWMGYELHPDKWTVQPIVLPSWTVNDIQKLVGKLNWASQIYP
GIKVRQLSKLLRGTKALTEVIPLTEEAELELAENREILKEPVHGVYYDPS
KDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMGAHTNDVKQLTEAVQ
KITTESIVIWGKTPKFKLPIQKETWETWWTEYWQATWIPEWEFVNTPPLV
KLWYQLEKEPIVGAETFYVDGAANRETKLGKAGYVTNKGRQKVVPLTNTT
NQKTELQAIYLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVNQIIE
QLIKKEKVYLAWVPAHKGIGGNEQVDKLVSAGI

3D structure

• PDB: 8sts Exploring Novel HIV-1 Reverse Transcriptase Inhibitors with Drug Resistant Mutants: A Double Mutant Surprise.
• Chain: A
• Resolution: 3.02 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.-
  2.7.7.49: RNA-directed DNA polymerase.
  2.7.7.7: DNA-directed DNA polymerase.
  3.1.-.-
  3.1.13.2: exoribonuclease H.
  3.1.26.13: retroviral ribonuclease H.
  3.4.23.16: HIV-1 retropepsin.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	7N1	A	L100 K101 K103 V179 Y188 W229 H235 P236 Y318	L87 K88 K90 V160 Y169 W210 H216 P217 Y296
BS02	MG	A	D443 D549	D420 D526

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003964 RNA-directed DNA polymerase activity
• GO:0004523 RNA-DNA hybrid ribonuclease activity

Biological Process

• GO:0006278 RNA-templated DNA biosynthetic process

External links

• PDB: RCSB:8sts, PDBe:8sts, PDBj:8sts
• PDBsum: 8sts
• PubMed: 37861472
• UniProt: P03366|POL_HV1B1 Gag-Pol polyprotein (Gene Name=gag-pol)