Structure of PDB 8srf Chain A

Receptor sequence

>8srfA (length=1377) Species: 946362 (Salpingoeca rosetta)

SVAAKTLLIENEDGKGSTRMEVQDFMKRFHMHASEDDKTGSPSTAWGTLR
FPTKEATAPYLRLSVNDDPEDALLFVKAMLAQKYGETYDRPSLILSVTGG
ARNFTLPPRLETAIAKGLRLAAQRTNAWVVTGGTNTGVMKLTGQIMEALS
KTQSHFIPPTIGIATYGVIIGGDDMTRGEPPKIGLEYEMHKKDPPKTTPL
DDNHNLFLLVDDGSTNKFGKEIKFRAAFENAAGQAFAAPVVTIVVQGGPG
TLGTALQAVRQGTPIVVVDGSGLAADVLAYAYNFMHNPLTRFKSYTIDDL
RQKVAQTFNPKSSQQLTNLLDSALECVQDPNLVVVYSLQESGIDEFDDCI
LKAIFSSQGKLGNKLKQAMYFDQLDVAKRALSEASKNGQHNEIAACINDN
LMAAMMHNKPHFVELYLGFDAKIYELKPSEEVAKTNITALDELPSFALAI
EELYKREAKKPHSHVQRLVSLSNTDVLGRHYRGRDLANTRAYNVLRMDQI
FARLVSKDFSVNRDFTIYDSKYDKVPGIQFRRTAQASHMLFLWAICLDRF
RMARHFWLIGDQSIINALVASRILERLSTHRALQGPHLAEERAKMQHNAK
KFEELAVGVLGECHGSDSHMASEMLHSKNDMFNKKNAINIAYDAKSLAFL
SHPATQSVINADWYGHLKSVTSFWAVLFAFFFPFFVLPFINFSGAHRLRR
KFAKFYSAPYTRFISDLLSHFVLCVVTSYFVLDKLEDTISAIEWILLVWF
VALLLEELRQMIFCDGIAEYISDTWNRLDLIMITLFFVGFFTHASDPSNQ
DSKVVSKGIHAFLVVVLWLRFMRYYALSKNLGPKLIMMMEMMKDVSTFVF
LLLIFLIGYGVAAQSLLSPDEDFSSRTFIGVLFRPYFQIYGELFLDDLNS
EANCLGDTPFTECSRETVRMVPFFLAVYILGSNVLLVNLLIAMFNDTYMK
VQEAAEDLWRKQNYELCAEYKDRPFLPAPFILLAHVHMLFMRLLRLCGVH
TQEHEKIQDDETKRKITTFEELNTDKFLRRWERERQEMLEARVKMTNDNV
VQAMGMMDQLLEHMISFRFSLDQQATKIPINRLNSAVAVHGHTAEAAEWY
VPPEEYPKSGGVKRYLIDASMVPLSIMCPSYDPVEYTHPSVAAQPVWADP
ADPRKIKFNVKDEVNGKVVDRTSCHPSGISIDSNTGRPINPWGRTGMTGR
GLLGKWGVNQAADTVVTRWKRSPDGSILERDGKKVLEFVAIQRQDNKMWA
IPGGFVDNGEDVALTSGREFMEEALGMGTSADLMSAESKDSLAALFSSGT
IVARIYCEDPRNTDNAWVETTCVNFHDESGRHAARLKLQGGDDAEHARWM
MVHGGLNLFASHRTLLQHVTSALNAYF

3D structure

• PDB: 8srf Coupling enzymatic activity and gating in an ancient TRPM chanzyme and its molecular evolution
• Chain: A
• Resolution: 2.52 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	RP5	A	D1330 R1360 F1372 C1424 D1426 R1428 F1476 H1479	D1213 R1243 F1255 C1307 D1309 R1311 F1359 H1362
BS02	AMP	A	W1264 G1321 N1326 F1372 E1386 D1460	W1147 G1204 N1209 F1255 E1269 D1343
BS03	APR	A	T148 G149 G150 A151 R152 N153 T184 T186 M189 F268 E271 R275 G297 G298 G300	T98 G99 G100 A101 R102 N103 T134 T136 M139 F218 E221 R225 G247 G248 G250
BS04	MG	A	E850 Q853 N869 D872	E757 Q760 N776 D779
BS05	MG	A	L621 I622 G623 E1114	L558 I559 G560 E1021
BS06	MG	A	E1390 D1460	E1273 D1343
BS07	MG	A	E1386 E1390 D1460	E1269 E1273 D1343
BS08	CLR	A	L878 V898 F905	L785 V805 F812
BS09	CLR	A	F1003 R1012 P1015 F1016	F910 R919 P922 F923
BS10	CLR	A	E1009 M1013 F1016	E916 M920 F923
BS11	CLR	A	F976 Y979	F883 Y886

Gene Ontology

Molecular Function

• GO:0005216 monoatomic ion channel activity
• GO:0005261 monoatomic cation channel activity
• GO:0005262 calcium channel activity

Biological Process

• GO:0006811 monoatomic ion transport
• GO:0055085 transmembrane transport
• GO:0070588 calcium ion transmembrane transport
• GO:0098655 monoatomic cation transmembrane transport

Cellular Component

• GO:0005886 plasma membrane
• GO:0016020 membrane

External links

• PDB: RCSB:8srf, PDBe:8srf, PDBj:8srf
• PDBsum: 8srf
• UniProt: F2UB89