Structure of PDB 8sre Chain A

Receptor sequence

>8sreA (length=1377) Species: 946362 (Salpingoeca rosetta)

SVAAKTLLIENEDGKGSTRMEVQDFMKRFHMHASEDDKTGSPSTAWGTLR
FPTKEATAPYLRLSVNDDPEDALLFVKAMLAQKYGETYDRPSLILSVTGG
ARNFTLPPRLETAIAKGLRLAAQRTNAWVVTGGTNTGVMKLTGQIMEALS
KTQSHFIPPTIGIATYGVIIGGDDMTRGEPPKIGLEYEMHKKDPPKTTPL
DDNHNLFLLVDDGSTNKFGKEIKFRAAFENAAGQAFAAPVVTIVVQGGPG
TLGTALQAVRQGTPIVVVDGSGLAADVLAYAYNFMHNPLTRFKSYTIDDL
RQKVAQTFNPKSSQQLTNLLDSALECVQDPNLVVVYSLQESGIDEFDDCI
LKAIFSSQGKLGNKLKQAMYFDQLDVAKRALSEASKNGQHNEIAACINDN
LMAAMMHNKPHFVELYLGFDAKIYELKPSEEVAKTNITALDELPSFALAI
EELYKREAKKPHSHVQRLVSLSNTDVLGRHYRGRDLANTRAYNVLRMDQI
FARLVSKDFSVNRDFTIYDSKYDKVPGIQFRRTAQASHMLFLWAICLDRF
RMARHFWLIGDQSIINALVASRILERLSTHRALQGPHLAEERAKMQHNAK
KFEELAVGVLGECHGSDSHMASEMLHSKNDMFNKKNAINIAYDAKSLAFL
SHPATQSVINADWYGHLKSVTSFWAVLFAFFFPFFVLPFINFSGAHRLRR
KFAKFYSAPYTRFISDLLSHFVLCVVTSYFVLDKLEDTISAIEWILLVWF
VALLLEELRQMIFCDGIAEYISDTWNRLDLIMITLFFVGFFTHASDPSNQ
DSKVVSKGIHAFLVVVLWLRFMRYYALSKNLGPKLIMMMEMMKDVSTFVF
LLLIFLIGYGVAAQSLLSPDEDFSSRTFIGVLFRPYFQIYGELFLDDLNS
EANCLGDTPFTECSRETVRMVPFFLAVYILGSNVLLVNLLIAMFNDTYMK
VQEAAEDLWRKQNYELCAEYKDRPFLPAPFILLAHVHMLFMRLLRLCGVH
TQEHEKIQDDETKRKITTFEELNTDKFLRRWERERQEMLEARVKMTNDNV
VQAMGMMDQLLEHMISFRFSLDQQATKIPINRLNSAVAVHGHTAEAAEWY
VPPEEYPKSGGVKRYLIDASMVPLSIMCPSYDPVEYTHPSVAAQPVWADP
ADPRKIKFNVKDEVNGKVVDRTSCHPSGISIDSNTGRPINPWGRTGMTGR
GLLGKWGVNQAADTVVTRWKRSPDGSILERDGKKVLEFVAIQRQDNKMWA
IPGGFVDNGEDVALTSGREFMEEALGMGTSADLMSAESKDSLAALFSSGT
IVARIYCEDPRNTDNAWVETTCVNFHDESGRHAARLKLQGGDDAEHARWM
MVHGGLNLFASHRTLLQHVTSALNAYF

3D structure

• PDB: 8sre Coupling enzymatic activity and gating in an ancient TRPM chanzyme and its molecular evolution
• Chain: A
• Resolution: 2.52 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	APR	A	T148 G149 G150 A151 R152 N153 T184 M189 F268 G298 T301 T304	T98 G99 G100 A101 R102 N103 T134 M139 F218 G248 T251 T254
BS02	MG	A	E850 Q853 D872	E757 Q760 D779
BS03	MG	A	L621 G623 E1114	L558 G560 E1021
BS04	MG	A	G1370 E1390	G1253 E1273
BS05	MG	A	E1386 E1390	E1269 E1273
BS06	CLR	A	V898 F905	V805 F812
BS07	APR	A	W1264 G1321 D1330 R1360 G1370 G1371 F1372 C1424 D1426 R1428 F1476 H1479	W1147 G1204 D1213 R1243 G1253 G1254 F1255 C1307 D1309 R1311 F1359 H1362
BS08	CLR	A	F1003 R1012 P1015	F910 R919 P922
BS09	CLR	A	E1009 M1013 F1016	E916 M920 F923
BS10	CLR	A	V942 L975 F976 Y979	V849 L882 F883 Y886

Gene Ontology

Molecular Function

• GO:0005216 monoatomic ion channel activity
• GO:0005261 monoatomic cation channel activity
• GO:0005262 calcium channel activity

Biological Process

• GO:0006811 monoatomic ion transport
• GO:0055085 transmembrane transport
• GO:0070588 calcium ion transmembrane transport
• GO:0098655 monoatomic cation transmembrane transport

Cellular Component

• GO:0005886 plasma membrane
• GO:0016020 membrane

External links

• PDB: RCSB:8sre, PDBe:8sre, PDBj:8sre
• PDBsum: 8sre
• UniProt: F2UB89