Structure of PDB 8src Chain A

Receptor sequence

>8srcA (length=1366) Species: 946362 (Salpingoeca rosetta)

VAAKTLLIENEDGKGSTRMEVQDFMKRFHMHASEDDKTGSPSTAWGTLRF
PTKEATAPYLRLSVNDDPEDALLFVKAMLAQKYGETYDRPSLILSVTGGA
RNFTLPPRLETAIAKGLRLAAQRTNAWVVTGGTNTGVMKLTGQIMEALSK
TQSHFIPPTIGIATYGVIIGGDDMTRGEPPKIGLEYEMHKKDPPKTTPLD
DNHNLFLLVDDGSTNKFGKEIKFRAAFENAAGQAFAAPVVTIVVQGGPGT
LGTALQAVRQGTPIVVVDGSGLAADVLAYAYNFMHNPLTRFKSYTIDDLR
QKVAQTFNPKSSQQLTNLLDSALECVQDPNLVVVYSLQESGIDEFDDCIL
KAIFSSQGKLGNKLKQAMYFDQLDVAKRALSEASKNGQHNEIAACINDNL
MAAMMHNKPHFVELYLGFDAKIYELKPSEEVAKTNITALDELPSFALAIE
ELYKREAKKPHSHVQRLVSLSNTDVLGRHYRLANTRAYNVLRMDQIFARL
VSKDFSVNRDFTIYDSKYDKVPGIQFRRTAQASHMLFLWAICLDRFRMAR
HFWLIGDQSIINALVASRILERLSTHRALQGPHLAEERAKMQHNAKKFEE
LAVGVLGECHGSDSHMASEMLHSKNDMFNKKNAINIAYDAKSLAFLSHPA
TQSVINADWYGHLKSVTSFWAVLFAFFFPFFVLPFINFSGAHRLRRKFAK
FYSAPYTRFISDLLSHFVLCVVTSYFVLDKLEDTISAIEWILLVWFVALL
LEELRQMIFCDGIAEYISDTWNRLDLIMITLFFVGFFTHASDPSNQDSKV
VSKGIHAFLVVVLWLRFMRYYALSKNLGPKLIMMMEMMKDVSTFVFLLLI
FLIGYGVAAQSLLSPDEDFSSRTFIGVLFRPYFQIYGELFLDDLNSEANC
LGDTPFTECSRETVRMVPFFLAVYILGSNVLLVNLLIAMFNDTYMKVQEA
AEDLWRKQNYELCAEYKDRPFLPAPFILLAHVHMLFMRLLRLCGVHTQEH
EKIQDDETKRKITTFEELNTDKFLRRWERERQEMLEARVKMTNDNVVQAM
GMMDQLLEHMISFRFSLDQQANRLNSAVAVHGHTAEAAEWYVPPEEYPKS
GGVKRYLIDASMVPLSIMCPSYDPVEYTHPSVAAQPVWADPADPRKIKFN
VKDEVNGKVVDRTSCHPSGISIDSNTGRPINPWGRTGMTGRGLLGKWGVN
QAADTVVTRWKRSPDGSILERDGKKVLEFVAIQRQDNKMWAIPGGFVDNG
EDVALTSGREFMEEALGMGTDLMSAESKDSLAALFSSGTIVARIYCEDPR
NTDNAWVETTCVNFHDESGRHAARLKLQGGDDAEHARWMMVHGGLNLFAS
HRTLLQHVTSALNAYF

3D structure

• PDB: 8src Coupling enzymatic activity and gating in an ancient TRPM chanzyme and its molecular evolution
• Chain: A
• Resolution: 2.92 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	APR	A	T148 G149 A151 R152 T184 F268 G298 G300 T301	T97 G98 A100 R101 T133 F217 G247 G249 T250
BS02	CA	A	E850 Q853 N869 D872	E753 Q756 N772 D775
BS03	CA	A	L621 I622 G623 E1114	L554 I555 G556 E1017
BS04	CA	A	G1370 E1390 D1460	G1244 E1264 D1332
BS05	CA	A	E1386 E1390 D1460	E1260 E1264 D1332
BS06	CLR	A	V898 F905	V801 F808
BS07	APR	A	W1264 F1372 R1428 D1460 F1476 H1479	W1138 F1246 R1300 D1332 F1348 H1351
BS08	CLR	A	R1012 P1015 F1016	R915 P918 F919
BS09	CLR	A	E1009 M1013 F1016	E912 M916 F919
BS10	CLR	A	F976 Y979	F879 Y882

Gene Ontology

Molecular Function

• GO:0005216 monoatomic ion channel activity
• GO:0005261 monoatomic cation channel activity
• GO:0005262 calcium channel activity

Biological Process

• GO:0006811 monoatomic ion transport
• GO:0055085 transmembrane transport
• GO:0070588 calcium ion transmembrane transport
• GO:0098655 monoatomic cation transmembrane transport

Cellular Component

• GO:0005886 plasma membrane
• GO:0016020 membrane

External links

• PDB: RCSB:8src, PDBe:8src, PDBj:8src
• PDBsum: 8src
• PubMed: 38773335
• UniProt: F2UB89