Structure of PDB 8sqm Chain A

Receptor sequence

>8sqmA (length=1398) Species: 4932 (Saccharomyces cerevisiae)

CKLCRSPEGFGPISFYGDFTQCFIDGVILNLSAIFMITFGIRDLVNLCKK
KHSGIKYRRNWIIVSRMALVLLEIAFVSLASLNISKEEAENFTIVSQYAS
TMLSLFVALALHWIEYDRSVVANTVLLFYWLFETFGNFAKLINILIRHTY
EGIWYSGQTGFILTLFQVITCASILLLEALPKKPLMPTRRKPNPYDSANI
FSRITFSWMSGLMKTGYEKYLVEADLYKLPRNFSSEELSQKLEKNWENEL
KQKSNPSLSWAICRTFGSKMLLAAFFKAIHDVLAFTQPQLLRILIKFVTD
YNSERQDPQKLPIVRGFLIAFAMFLVGFTQTSVLHQYFLNVFNTGMYIKS
ALTALIYQKSLVLSNEASGLSSTGDIVNLMSVDVQKLQDLTQWLNLIWSG
PFQIIICLYSLYKLLGNSMWVGVIILVIMMPLNSFLMRIQKKLQKSQMKY
KDERTRVISEILNNIKSLKLYAWEKPYREKLEEVRNNKELKNLTKLGCYM
AVTSFQFNIVPFLVSCCTFAVFVYTEDRALTTDLVFPALTLFNLLSFPLM
IIPMVLNSFIEASVSIGRLFTFFTNEELQPDSVQRLPKVKNIGDVAINIG
DDATFLWQRKPEYKVALKNINFQAKKGNLTCIVGKVGSGKTALLSCMLGD
LFRVKGFATVHGSVAYVSQVPWIMNGTVKENILFGHRYDAEFYEKTIKAC
ALTIDLAILMDGDKTLVGEKGISLSGGQKARLSLARAVYARADTYLLDDP
LAAVDEHVARHLIEHVLGPNGLLHTKTKVLATNKVSALSIADSIALLDNG
EITQQGTYDEITKDADSPLWKLLREHREQGKVKWNIYLEYAKACNPKSVC
VFILFIVISMFLSVMGNVWLKHWSEVNSRYGSNPNAARYLAIYFALGIGS
ALATLIQTIVLWVFCTIHASKYLHNLMTNSVLRAPMTFFETTPIGRILNR
FSNDIYKVDALLGRTFSQFFVNAVKVTFTITVICATTWQFIFIIIPLSVF
YIYYQQYYLRTSRELRRLDSITRSPIYSHFQETLGGLATVRGYSQQKRFS
HINQCRIDNNMSAFYPSINANRWLAYRLELIGSIIILGAATLSVFRLKQG
TLTAGMVGLSLSYALQITQTLNWIVRMTVEVETNIVSVERIKEYADLKSE
APLIVEGHRPPGDIKFNNYSTRYRPELDLVLKHINIHIKPNEKVGIVGRT
GAGKSSLTLALFRMIEASEGNIVIDNIAINEIGLYDLRHKLSIIPQDSQV
FEGTVRENIDPINQYTDEAIWRALELSHLKEHVLSMSNDGLDAQLTEGGG
NLSVGQRQLLCLARAMLVPSKILVLDEATAAVDVETDKVVQETIRTAFKD
RTILTIAHRLNTIMDSDRIIVLDNGKVAEFDSPGQLLSDNKSLFYSLC

3D structure

• PDB: 8sqm Structure of a dimeric ABC transporter
• Chain: A
• Resolution: 3.4 Å

Enzymatic activity

• Enzyme Commision number: 7.2.2.2: ABC-type Cd(2+) transporter.
  7.6.2.3: ABC-type glutathione-S-conjugate transporter.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	A	E240 G714 H715	E223 G685 H686

Gene Ontology

Molecular Function

• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0008551 P-type cadmium transporter activity
• GO:0015127 bilirubin transmembrane transporter activity
• GO:0015431 ABC-type glutathione S-conjugate transporter activity
• GO:0015434 ABC-type cadmium transporter activity
• GO:0016887 ATP hydrolysis activity
• GO:0042626 ATPase-coupled transmembrane transporter activity
• GO:0140359 ABC-type transporter activity

Biological Process

• GO:0006749 glutathione metabolic process
• GO:0010038 response to metal ion
• GO:0015723 bilirubin transport
• GO:0042144 vacuole fusion, non-autophagic
• GO:0045454 cell redox homeostasis
• GO:0046686 response to cadmium ion
• GO:0055085 transmembrane transport
• GO:0070574 cadmium ion transmembrane transport

Cellular Component

• GO:0000329 fungal-type vacuole membrane
• GO:0005773 vacuole
• GO:0005774 vacuolar membrane
• GO:0016020 membrane
• GO:0045121 membrane raft

External links

• PDB: RCSB:8sqm, PDBe:8sqm, PDBj:8sqm
• PDBsum: 8sqm
• UniProt: P39109|YCFI_YEAST Metal resistance protein YCF1 (Gene Name=YCF1)