Structure of PDB 8spq Chain A

Receptor sequence
>8spqA (length=1341) Species: 301447 (Streptococcus pyogenes serotype M1) [Search protein sequence]
KKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALL
FDSGETAERTRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLR
LIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPIN
ASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNF
KSNFDLAEDAKLQLSKDTYDDDLDNLLAQIDLFLAAKNLSDAILLSDILR
VNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKN
GYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDN
GSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLAR
GNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKV
LPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNR
KVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFL
DNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYT
GWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKED
IQKAQDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIE
MARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDK
NRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELD
KAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKL
VSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGD
YKVIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWD
KGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESIRPKRNSDKLIARKKD
WDPKKYGGFLWPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFE
KNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAKQLQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISE
FSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTRLGAPRAFK
YFDTTIDPKQYRSTKEVLDATLIHQSITGLYETRIDLSQLG
3D structure
PDB8spq Unraveling the mechanisms of PAMless DNA interrogation by SpRY-Cas9
ChainA
Resolution3.26 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.-.-
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 rna A K31 K33 L35 I43 K44 N46 E57 T58 A59 R61 T62 R63 K65 R66 R69 R70 R71 Y72 R74 R75 K76 N77 L101 S104 F105 R115 H116 I122 H129 T134 I135 H160 K163 F164 R165 G166 Y325 H328 H329 L332 K336 R340 F351 F352 Y359 A360 I363 Q402 R403 T404 F405 N407 G408 F446 R447 I448 Y450 G453 P454 L455 A456 R457 N459 S460 F462 T472 I473 P475 F491 K510 Y515 N588 R661 H721 S730 P731 K735 L738 K742 K749 R753 R765 S777 E779 R780 N803 D849 D850 S851 I852 K918 R919 T957 Y1010 Y1013 P1090 E1099 V1100 T1102 G1103 G1104 F1105 I1110 P1112 K1113 R1122 K1123 K1124 K1130 Y1131 E1225 A1227 Q1272 H1349 Q1350 S1351 Y1356 K29 K31 L33 I41 K42 N44 E55 T56 A57 R59 T60 R61 K63 R64 R67 R68 R69 Y70 R72 R73 K74 N75 L99 S102 F103 R113 H114 I120 H127 T132 I133 H158 K161 F162 R163 G164 Y318 H321 H322 L325 K329 R333 F344 F345 Y352 A353 I356 Q395 R396 T397 F398 N400 G401 F439 R440 I441 Y443 G446 P447 L448 A449 R450 N452 S453 F455 T465 I466 P468 F484 K503 Y508 N581 R654 H709 S718 P719 K723 L726 K730 K737 R741 R753 S765 E767 R768 N791 D837 D838 S839 I840 K906 R907 T945 Y998 Y1001 P1065 E1074 V1075 T1077 G1078 G1079 F1080 I1085 P1087 K1088 R1097 K1098 K1099 K1105 Y1106 E1200 A1202 Q1247 H1324 Q1325 S1326 Y1331
BS02 dna A S845 N863 R864 S867 D868 K1107 E1108 S1109 W1136 S833 N851 R852 S855 D856 K1082 E1083 S1084 W1111
BS03 dna A D10 N14 S15 G915 F916 Q920 L921 H983 Y1001 K1003 K1014 R1058 R1114 K1118 L1135 W1136 P1137 S1216 Q1219 Q1221 A1320 R1322 D8 N12 S13 G903 F904 Q908 L909 H971 Y989 K991 K1002 R1033 R1089 K1093 L1110 W1111 P1112 S1191 Q1194 Q1196 A1295 R1297
BS04 dna A Y450 M495 N497 K526 G582 N588 R655 T657 W659 R661 N692 M694 Q695 R765 E766 N767 Q768 T770 Q774 K810 S834 D837 V838 D839 H840 N863 R925 Q926 K929 Y1013 A1032 Y443 M488 N490 K519 G575 N581 R648 T650 W652 R654 N685 M687 Q688 R753 E754 N755 Q756 T758 Q762 K798 S822 D825 V826 D827 H828 N851 R913 Q914 K917 Y1001 A1007
BS05 MG A D10 H983 D8 H971
BS06 MG A D839 N863 D827 N851
Gene Ontology
Molecular Function
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0004519 endonuclease activity
GO:0004520 DNA endonuclease activity
GO:0004527 exonuclease activity
GO:0008408 3'-5' exonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0043571 maintenance of CRISPR repeat elements
GO:0051607 defense response to virus

View graph for
Molecular Function
View graph for
Biological Process
External links
PDB RCSB:8spq, PDBe:8spq, PDBj:8spq
PDBsum8spq
PubMed38688943
UniProtQ99ZW2|CAS9_STRP1 CRISPR-associated endonuclease Cas9/Csn1 (Gene Name=cas9)

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