Structure of PDB 8snx Chain A

Receptor sequence

>8snxA (length=1386) Species: 1972429 (Respiratory syncytial virus A2)

ANVYLTDSYLKGVISFSECNALGSYIFNGPYLKNDYTNLISRQNPLIEHM
NLKKLNITQSLISKYHKGEIKLEEPTYFQSLLMTYKSMTSSEQIATTNLL
KKIIRRAIEISDVKVYAILNKLGLTIKTTLLKKLMCSMQHPPSWLIHWFN
LYTKLNNILTQYRSNEVKNHGFTLIDNQTLSGFQFILNQYGCIVYHKELK
RITVTTYNQFLTWKDISLSRLNVCLITWISNCLNTLNKSLGLRCGFNNVI
LTQLFLYGDCILKLFHNEGFYIIKEVEGFIMSLILNITEEDQFRKRFYNS
MLNNITDAANKAQKNLLSRVCHTLLDKTVSDNIINGRWIILLSKFLKLIK
LAGDNNLNNLSELYFLFRIFGHPMVDERQAMDAVKINCNETKFYLLSSLS
MLRGAFIYRIIKGFVNNYNRWPTLRNAIVLPLRWLTYYKLNTYPSLLELT
ERDLIVLSGLRFYREFRLPKKVDLEMIINDKAISPPKNLIWTSFPRNYMP
SHIQNYIEHEKLKFSESDKSRRVLEYYLRDNKFNECDLYNCVVNQSYLNN
PNHVVSLTGKERELSVGRMFAMQPGMFRQVQILAEKMIAENILQFFPESL
TRELQKILELKAGNYISKCSIITDLSKFNQAFRYETSCICSDVLDELHGV
QSLFSWLHLTIPHVTIICTYRHAPPYIGDHIVDLNNVDEQSGLYRYHMGG
IEGWCQKLWTIEAISLLDLISLKGKFSITALINGDNQSIDISKPIRLMEG
QTHAQADYLLALNSLKLLYKEYAGIGHKLKGTETYISRDMQFMSKTIQHN
GVYYPASIKKVLRVGPWINTILDDFKVSLESIGSLTQELEYRGESLLCSL
IFRNVWLYNQIALQLKNHALCNNKLYLDILKVLKHLKTFFNLDNIDTALT
LYMNLPMLFGGGDPNLLYRSFYRRTPDFLTEAIVHSVFILSYYTNHDLKD
KLQDLSDDRLNKFLTCIITFDKNPNAEFVTLMRDPQALGSERQAKITSEI
NRLAVTEVLSTAPNKIFSKSAQHYTTTEIDLNDIMQNIEPTYPHGLRVVY
ESLPFYKAEKIVNLISGTKSITNILEKTSAIDLTDIDRATEMMRKNITLL
IRILPLDCNRDKREILSMENLSITELSKYVRERSWSLSNIVGVTSPSIMY
TMDIKYTTSTISSGIIIEKYNVNSLTRGERGPTKPWVGSSTQEKKTMPVY
NRQVLTKKQRDQIDLLAKLDWVYASIDNKDEFMEELSIGTLGLTYEKAKK
LFPQYLSVNYLHRLTVSSRPCEFPASIPAYRTTNYHFDTSPINRILTEKY
GDEDIDIVFQNCISFGLSLMSVVEQFTNVCPNRIILIPKLNEIHLMKPPI
FTGDVDIHKLKQVIQKQHMFLPDKISLTQYVELFLS

3D structure

• PDB: 8snx Structures of the promoter-bound respiratory syncytial virus polymerase
• Chain: A
• Resolution: 3.4 Å

Enzymatic activity

• Enzyme Commision number: 2.1.1.375: NNS virus cap methyltransferase.
  2.7.7.48: RNA-directed RNA polymerase.
  2.7.7.88: GDP polyribonucleotidyltransferase.
  3.6.1.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	Y13 E57 Y249 K540 A541 T551 F553 P554 R555 I566 S579 R580 R581 E584 K619 E620 F629 A630 M631 R637 Q640 T660 R747 E778 G779 K783 K1153 S1155	Y4 E48 Y190 K481 A482 T492 F494 P495 R496 I507 S520 R521 R522 E525 K560 E561 F570 A571 M572 R578 Q581 T601 R671 E702 G703 K707 K1077 S1079

Gene Ontology

Molecular Function

• GO:0003924 GTPase activity
• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0004482 mRNA 5'-cap (guanine-N7-)-methyltransferase activity
• GO:0005524 ATP binding
• GO:0008168 methyltransferase activity
• GO:0016740 transferase activity
• GO:0016787 hydrolase activity
• GO:0034062 5'-3' RNA polymerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0001172 RNA-templated transcription
• GO:0006139 nucleobase-containing compound metabolic process
• GO:0006370 7-methylguanosine mRNA capping
• GO:0032259 methylation
• GO:0039689 negative stranded viral RNA replication
• GO:0106005 RNA 5'-cap (guanine-N7)-methylation

Cellular Component

• GO:0030430 host cell cytoplasm
• GO:0044423 virion component

External links

• PDB: RCSB:8snx, PDBe:8snx, PDBj:8snx
• PDBsum: 8snx
• PubMed: 38123676
• UniProt: P28887|L_HRSVA RNA-directed RNA polymerase L (Gene Name=L)