Structure of PDB 8sl9 Chain A

Receptor sequence

>8sl9A (length=422) Species: 177416 (Francisella tularensis subsp. tularensis SCHU S4)

GMQYIIGIGTNIGFTIENIHLAITALESQQNIRIIRKASLYSSKAVLKED
APKEWDIRFLNTAVKISSSLKPDELLVLLKDIELKIGRDLNAPAWSPRVI
DLDILAAEDLILETDKLTIPHKELINRSFALAPLLELSKGWHHPKYVEWD
LNIRLKELGEIVKLKQTLANTIRMGIVNLSNQSFSDGNFDDNQRKLNLDE
LIQSGAEIIDIGAESTKPDAKPISIEEEFNKLDEFLEYFKSQLANLIYKP
LVSIDTRKLEVMQKILAKHHDIIWMINDVECNNIEQKAQLIAKYNKKYVI
IHNLGITDRNQYLDKENAIDNVCDYIEQKKQILLKHGIAQQNIYFDIGFG
FGKKSDTARYLLENIIEIKRRLELKALVGHSRKPSVLGLAKDSNLATLDR
ATRELSRKLEKLDIDIIRVHKI

3D structure

• PDB: 8sl9 Crystal structure of Francisella tularensis HPPK-DHPS in complex with HPPK inhibitor HP-73
• Chain: A
• Resolution: 1.4 Å

Enzymatic activity

• Enzyme Commision number: 2.5.1.15: dihydropteroate synthase.
  2.7.6.3: 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	H73	A	K43 V45 F58 N60 L75 K79 R97 D100 D102 I103 K115 L116 T117 H120 R126 F128	K44 V46 F59 N61 L76 K80 R98 D101 D103 I104 K116 L117 T118 H121 R127 F129

Gene Ontology

Molecular Function

• GO:0003848 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity
• GO:0004156 dihydropteroate synthase activity
• GO:0005524 ATP binding
• GO:0016301 kinase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0009396 folic acid-containing compound biosynthetic process
• GO:0016310 phosphorylation
• GO:0042558 pteridine-containing compound metabolic process
• GO:0044237 cellular metabolic process
• GO:0046654 tetrahydrofolate biosynthetic process
• GO:0046656 folic acid biosynthetic process

Cellular Component

• GO:0005829 cytosol

External links

• PDB: RCSB:8sl9, PDBe:8sl9, PDBj:8sl9
• PDBsum: 8sl9
• UniProt: Q5NGA7