Structure of PDB 8sfr Chain A

Receptor sequence

>8sfrA (length=1240) Species: 1111120 (Acidaminococcus sp. BV3L6) [Search protein sequence]

MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDHYKEL
KPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQA
TYRNAIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVT
TTEHENALLRSFDKFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPK
FKENCHIFTRLITAVPSLREHFENVKKAIGIFVSTSIEEVFSFPFYNQLL
TQTQIDLYNQLLGGISREAGTEKIKGLNEVLNLAIQKNDETAHIIASLPH
RFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLRNENVLETAE
ALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELKSA
KEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLP
TTLKKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKL
EMEPSLSFYNKARNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAI
LFVKNGLYYLGIMPKQKGRYKALSFEPTEKTSEGFDKMYYDYFPDAAKMI
PKCSTQLKAVTAHFQTHTTPILLSNNFIEPLEITKEIYDLNNPEKEPKKF
QTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDLSSLRPSSQYK
DLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGHH
GKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSHEIIKDRRFTSD
KFFFHVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYI
TVIDSTGKILEQRSLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDL
KQGYLSQVIHEIVDLMIHYQAVVVLENLNFGFKSKRTGIAEKAVYQQFEK
MLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQFTSFAKMGTQSGFLFYVPA
PYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDVKTGDFILHF
KMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH
RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVA
LIRSVLQMRNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGA
YHIALKGQLLLNHLKESKDLKLQNGISNQDWLAYIQELRN

3D structure

PDB

8sfr WT CRISPR-Cas12a post nontarget strand cleavage.

Chain

Resolution

3.5 Å

3D
structure

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Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	S14 K15 T16 R18 K51 N175 R176 T187 R192 T271 Q286 F306 K307 I309 L310 W382 K414 H479 L511 Y514 N515 R518 N747 K748 G753 H754 H755 G756 K757 N759 L760 H761 H856 I858 I859 K860 R862 R863 H872 Q936 F938 R955 D966 L967 Q969 G970 S973 Q974 H977 K1022 K1029	S14 K15 T16 R18 K51 N175 R176 T187 R192 T271 Q286 F306 K307 I309 L310 W382 K409 H474 L506 Y509 N510 R513 N742 K743 G748 H749 H750 G751 K752 N754 L755 H756 H789 I791 I792 K793 R795 R796 H805 Q869 F871 R888 D899 L900 Q902 G903 S906 Q907 H910 K955 K962
BS02	dna	A	K164 N178 I185 S186 G263 S266 N278 E279 N282 Q286 R301 E372 W382 N519 T522 K523 K524 G543 D545 N547 K548 Y597 P599 M604 K607 L612 K613 K689 N782 G783 Q784 K965 S1051 F1052	K164 N178 I185 S186 G263 S266 N278 E279 N282 Q286 R301 E372 W382 N514 T517 K518 K519 G538 D540 N542 K543 Y592 P594 M599 K602 L607 K608 K684 N777 G778 Q779 K898 S984 F985
BS03	dna	A	K134 A135 K164 F165 T166 T167 P538 K570 R574 Y575 A602 K603 K607 Q611 K653 Q656 T657 K661 Y711	K134 A135 K164 F165 T166 T167 P533 K565 R569 Y570 A597 K598 K602 Q606 K648 Q651 T652 K656 Y706

Gene Ontology

	Molecular Function
GO:0003677	DNA binding
GO:0003723	RNA binding
GO:0004519	endonuclease activity
GO:0004530	deoxyribonuclease I activity
GO:0016829	lyase activity

	Biological Process
GO:0006259	DNA metabolic process
GO:0051607	defense response to virus

View graph for
Molecular Function

View graph for
Biological Process

External links

PDB	RCSB:8sfr, PDBe:8sfr, PDBj:8sfr
PDBsum	8sfr
PubMed
UniProt	U2UMQ6\|CS12A_ACISB CRISPR-associated endonuclease Cas12a (Gene Name=cas12a)

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