Structure of PDB 8sea Chain A

Receptor sequence

>8seaA (length=992) Species: 9606 (Homo sapiens)

VLGSPAMQRIQGARVLVSGLQGLGAEVAKNLVLMGVGSLTLHDPHPTCWS
DLAAQFLLSEQDLERSRAEASQELLAQLNRAVQVVVHTGDITEDLLLDFQ
VVVLTAAKLEEQLKVGTLCHKHGVCFLAADTRGLVGQLFCDFGEDFTVQD
PTEAEPLTAAIQHISQGSPGILTLRKGANTHYFRDGDLVTFSGIEGMVEL
NDCDPRSIHVREDGSLEIGDTTTFSRYLRGGAITEVKRPKTVRHKSLDTA
LLQPHVVAQSSQEVHHAHCLHQAFCALHKFQHLHGRPPQPWDPVDAETVV
GLARDLEPLKRTEEEPLEEPLDEALVRTVALSSAGVLSPMVAMLGAVAAQ
EVLKAISRKFMPLDQWLYFDALDCLPEDGELLPSPEDCALRGSRYDGQIA
VFGAGFQEKLRRQHYLLVGAGAIGCELLKVFALVGLGAGNSGGLTVVDMD
HIERSNLSRQFLFRSQDVGRPKAEVAAAAARGLNPDLQVIPLTYPLDPTT
EHIYGDNFFSRVDGVAAALDSFQARRYVAARCTHYLKPLLEAGTSGTWGS
ATVFMPHVTEAYRAPASAAASEDAPYPVCTVRYFPSTAEHTLQWARHEFE
ELFRLSAETINHHQQAHTSLADMDEPQTLTLLKPVLGVLRVRPQNWQDCV
AWALGHWKLCFHYGIKQLLRHFPPNKVLEDGTPFWSGPKQCPQPLEFDTN
QDTHLLYVLAAANLYAQMHGLPGSQDWTALRELLKLLPQPDPQQMAPIFA
SNLELASASAEFGPEQQKELNKALEVWSVGPPLKPLMFEKDDDSNFHVDF
VVAAASLRCQNYGIPPVNRAQSKRIVGQIIPAIATTTAAVAGLLGLELYK
VVSGPRPRSAFRHSYLHLAENYLIRYMPFAPAIQTFHHLKWTSWDRLKVP
AGQPERTLESLLAHLQEQHGLRVRILLHGSALLYAAGWSPEKQAQHLPLR
VTELVQQLTGQAPAPGQRVLVLELSCEGDDEDTAFPPLHYEL

3D structure

• PDB: 8sea Cryo-EM structures of Uba7 reveal the molecular basis for ISG15 activation and E1-E2 thioester transfer.
• Chain: A
• Resolution: 3.4 Å

Enzymatic activity

• Enzyme Commision number: 6.2.1.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	AMP	A	G441 A442 D468 M469 K492 L516 L539 D540	G421 A422 D448 M449 K472 L496 L519 D520

Gene Ontology

Molecular Function

• GO:0004842 ubiquitin-protein transferase activity
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0008641 ubiquitin-like modifier activating enzyme activity
• GO:0016874 ligase activity
• GO:0019782 ISG15 activating enzyme activity

Biological Process

• GO:0006974 DNA damage response
• GO:0016567 protein ubiquitination
• GO:0019941 modification-dependent protein catabolic process
• GO:0032020 ISG15-protein conjugation
• GO:0032446 protein modification by small protein conjugation
• GO:0036211 protein modification process
• GO:0045087 innate immune response

Cellular Component

• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:8sea, PDBe:8sea, PDBj:8sea
• PDBsum: 8sea
• PubMed: 37553340
• UniProt: P41226|UBA7_HUMAN Ubiquitin-like modifier-activating enzyme 7 (Gene Name=UBA7)