Structure of PDB 8sdb Chain A

Receptor sequence

>8sdbA (length=600) Species: 562 (Escherichia coli)

THLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWDGRRHPM
RLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPET
ASLICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYR
ELADQLVPYAKWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRD
DFRYFIDAAHAAGLNVILDWVPGHFPTDDFALAEFDGTNLYEHSTLIYNY
GRREVSNFLVGNALYWIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEF
GGRENLEAIEFLRNTNRILGEQVSGAVTMAEESTDFPGVSRPQDMGGLGF
WYKWNLGWMHDTLDYMKLDPVYRQYHHDKLTFGILYNYTENFVLPLSHDE
VVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLLFMGNEFAQGRE
WNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPYGFE
WLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWRE
ILNTDSMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREA

3D structure

• PDB: 8sdb The Structure of Maltooctaose-Bound Escherichia coli Branching Enzyme Suggests a Mechanism for Donor Chain Specificity.
• Chain: A
• Resolution: 3.0 Å

Enzymatic activity

• Enzyme Commision number: 2.4.1.18: 1,4-alpha-glucan branching enzyme.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GLC	A	R255 S583 H587	R139 S456 H460
BS02	GLC	A	S583 L584	S456 L457
BS03	GLC	A	N690 G694	N563 G567
BS04	GLC	A	S683 M684 N690 G694	S556 M557 N563 G567
BS05	GLC	A	S683 H685 Y686	S556 H558 Y559
BS06	GLC	A	W159 K189	W43 K73
BS07	GLC	A	W159 Q211	W43 Q95
BS08	GLC	A	Y275 W278	Y159 W162
BS09	GLC	A	D537 K546	D410 K419

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0003844 1,4-alpha-glucan branching enzyme activity
• GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
• GO:0043169 cation binding

Biological Process

• GO:0005975 carbohydrate metabolic process
• GO:0005978 glycogen biosynthetic process

External links

• PDB: RCSB:8sdb, PDBe:8sdb, PDBj:8sdb
• PDBsum: 8sdb
• PubMed: 37298853
• UniProt: P07762|GLGB_ECOLI 1,4-alpha-glucan branching enzyme GlgB (Gene Name=glgB)