Structure of PDB 8s9t Chain A

Receptor sequence

>8s9tA (length=769) Species: 1148 (Synechocystis sp. PCC 6803)

MRGIEITITMQSDWHVGTGMGRGELDSVVQRDGDNLPYIPGKTLTGILRD
SCEQVALGLDNGQTRGLWHGWINFIFGDQPALAQGAIEPEPRPALIAIGS
AHLDPKLKAAFQGKKQLQEAIAFMKPGVAIDAITGTAKKDFLRFEEVVRL
GAKLTAEVELNLPDNLSETNKKVIAGILASGAKLTERLGGKRRRGNGRCE
LKFSGYSDQQIQWLKDNYQSVDQPPKYQQNKLQSAGDNPEQQPPWHIIPL
TIKTLSPVVLPARTVGNVVECLDYIPGRYLLGYIHKTLGEYFDVSQAIAA
GDLIITNATIKIDGKAGRATPFCLFGEKLDGGLGKGKGVYNRFQESEPDG
IQLKGERGGYVGQFEQEQRNLPNTGKINSELFTHNTIQDDVQRPTSDVGG
VYSYEAIIAGQTFVAELRLPDSLVKQITSKNKNWQAQLKATIRIGQSKKD
QYGKIEVTSGNSADLPKPTGNNKTLSIWFLSDILLRGDRLNFNATPDDLK
KYLENALDIKLKERSDNDLICIALRSQRTESWQVRWGLPRPSLVGWQAGS
CLIYDIESGTVNAEKLQELMITGIGDRCTEGYGQIGFNDPLLSASLGKLT
AKPSNPLPTNHPTQDYARLIEKAAWREAIQNKALALASSRAKREEILGIK
IMGKDSQPTMTQLGGFRSVLKRLHSRNNRDIVTGYLTALEQVSNRKEKWS
NTSQGLTKIRNLVTQENLIWNHLDIDFSPLTITQNGVNQLKSELWAEAVR
TLVDAIIRGHKRDLEKAQE

3D structure

• PDB: 8s9t RNA targeting and cleavage by the type III-Dv CRISPR effector complex.
• Chain: A
• Resolution: 2.52 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	V16 G17 T18 G19 K42 T43 G46 R49 D50 D78 Q79 P80 P93 A94 G127 V128 A129 I130 T136 A137 L142 R143 G189 G190 K191 R192 R193 R194 L232 P261 Y279 L281 H285 S295 I298 T383 H384 N385 T386 I387 Q392 R393 P394 V401 Y402 I444 G445 S447 K448 K449 W532 Q533 V534 R535 R540 R577 E580	V16 G17 T18 G19 K42 T43 G46 R49 D50 D78 Q79 P80 P93 A94 G127 V128 A129 I130 T136 A137 L142 R143 G189 G190 K191 R192 R193 R194 L232 P261 Y279 L281 H285 S295 I298 T383 H384 N385 T386 I387 Q392 R393 P394 V401 Y402 I444 G445 S447 K448 K449 W532 Q533 V534 R535 R540 R577 E580

Gene Ontology

Molecular Function

• GO:0003723 RNA binding

Biological Process

• GO:0051607 defense response to virus

External links

• PDB: RCSB:8s9t, PDBe:8s9t, PDBj:8s9t
• PDBsum: 8s9t
• PubMed: 38637512
• UniProt: Q6ZED2