Structure of PDB 8s8w Chain A

Receptor sequence

>8s8wA (length=299) Species: 2697049 (Severe acute respiratory syndrome coronavirus 2)

SQAWQPGVAMPNLYKMQRMLLEKCDLQNYGDSATLPKGIMMNVAKYTQLC
QYLNTLTLAVPYNMRVIHFGAGSDKGVAPGTAVLRQWLPTGTLLVDSDLN
DFVSDADSTLIGDCATVHTANKWDLIISDMYDPKTKNVTKENDSKEGFFT
YICGFIQQKLALGGSVAIKITEHSWNADLYKLMGHFAWWTAFVTNVNASS
SEAFLIGCNYLGKPREQIDGYVMHANYIFWRNTNPIQLSSYSLFDMSKFP
LKLRGTAVMSLKEGQINDMILSLLSKGRLIIRENNRVVISSDVLVNNEN

3D structure

• PDB: 8s8w SARS-CoV-2 methyltransferase nsp10-16 in complex with natural and drug-like purine analogs for guiding structure-based drug development
• Chain: A
• Resolution: 2.1 Å

Enzymatic activity

• Enzyme Commision number: 2.1.1.56: mRNA (guanine-N(7))-methyltransferase.
  2.1.1.57: methyltransferase cap1.
  2.7.7.48: RNA-directed RNA polymerase.
  2.7.7.50: mRNA guanylyltransferase.
  3.1.13.-
  3.4.19.12: ubiquitinyl hydrolase 1.
  3.4.22.-
  3.4.22.69: SARS coronavirus main proteinase.
  3.6.4.12: DNA helicase.
  3.6.4.13: RNA helicase.
  4.6.1.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	A6832 L6834 M6840 N6841 K6844 D6873 K6874	A33 L35 M41 N42 K45 D74 K75
BS02	SAM	A	N6841 Y6845 G6869 A6870 G6871 P6878 G6879 D6897 L6898 C6913 D6928 M6929 Y6930 F6947	N42 Y46 G70 A71 G72 P79 G80 D98 L99 C114 D129 M130 Y131 F148
BS03	SGV	A	D6897 L6898 D6912 C6913 D6928 M6929 Y6930 D6931	D98 L99 D113 C114 D129 M130 Y131 D132
BS04	GTA	A	C6823 L6825 Y6828 Y6930 K6935 K6968 T6970 E6971 H6972 S6999 S7000	C24 L26 Y29 Y131 K136 K169 T171 E172 H173 S200 S201

Gene Ontology

Molecular Function

• GO:0004483 mRNA (nucleoside-2'-O-)-methyltransferase activity

External links

• PDB: RCSB:8s8w, PDBe:8s8w, PDBj:8s8w
• PDBsum: 8s8w
• UniProt: P0DTD1|R1AB_SARS2 Replicase polyprotein 1ab (Gene Name=rep)