Structure of PDB 8qh3 Chain A

Receptor sequence

>8qh3A (length=1310) Species: 11602 (Hantaan virus 76-118)

YIPDYKGMEPIGANIEDLVDYSKDWLSRARNFSFFEVKGTAVFECFNSNE
ANHCQRYPMSRKPRNFLLIQCSLITSYKPATTLSDQIDSRRACSYILNLI
PDTPASYLIHDMAYRYINLTREDMINYYAPRIQFKQTQNVREPGTFKLTS
SMLRAESKAMLDLLNNQIESLNIASHIVQSESVSLITKILSDLELNIVLD
KFFQNETQKYLIDVLKKTTAWHIGHLIRDITESLIAHSGLKRSKYWSLHS
YNNGNVILFILPSKSLEVAGSFIRFITVFRIGPGLVDKDNLDTILIDGDS
QWGVSKVMSIDLNRLLALNIAFEKALIATATWFQYYTEDQGQFPLQYAIR
SVFANHFLLAICQKMKLCAIFDNLRYLIPAVTSLYSGFPSLIEKLFERPF
KSSLEVYIYYNIKSLLVALAQNVYPSFMSRIVYKHYRSLISEVTTCFFLF
EKGLHGNMNEEAKIHLETVEWALKFREKEEKYGESLVENGYMMWELRANA
ELAEQQLYCQDAIELAAIELNKVLATKSSVVANSILSKNWEEPYFSQTRN
ISLKGMSGQVQEDGHLSSSVTIIEAIRYLSNSRHNPSLLKLYEETREQKA
MARIVRKYQRTEADRGFFITTLPTRCRLEIIEDYYDAIAKNISEEYISYG
GEKKILAIQGALEKALRWASGESFIELSNHKFIRMKRKLMYVSADATKWS
PGDNSAKFRRFTSMLHNGLPNNKLKNCVIDALKQVYKTDFFMSRKLRNYI
DSMESLDPHIKQFLDFFPDGHHGEVKGNWLQGNLNKCSSLFGVAMSLLFK
QVWTNLFPELDCFFEFAHHSDDALFIYGYLEPVDDGTDWFLFVSQQIQAG
HLHWFSVNTEMWKSMFNLHEHILLLGSIKISPKKTTVSPTNAEFLSTFFE
GCAVSIPFVKILLGSLSDLPGLGYFDDLAAAQSRCVKALDLGASPQVAQL
AVALCTSKVERLYGTAPGMVNHPAAYLQVKHTDTPIPLGGNGAMSIMELA
TAGIGMSDKNLLKRALLGYSHKRQKSMLYILGLFKFLMKLSDETFQHERL
GQFSFIGKVQWKIFTPKSEFEFADMYTSKFLELWSSQHVTYDYIIPKGRD
NLLIYLVRKLNDPSIVTAMTMQSPLQLRFRMQAKQHMKVCRLDGEWVTFR
EVLAAANSFAENYSATSQDMDLFQTLTSCTFSKEYAWKDFLNGIHCDVIP
TKQVQRAKVARTFTSIPAVIGYKFAVTVEEMSDVLDTAKFPDSLSVDLKT
MKDGVYRELGLDISLPDVMKRIAPMLYKRVVIVQGNVEGTAEAICRYWLK
SMSLVKTIRV

3D structure

• PDB: 8qh3 Structural characterization of the oligomerization of full-length Hantaan virus polymerase into symmetric dimers and hexamers.
• Chain: A
• Resolution: 2.81 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.
  3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	Y282 L389 Q401 I402 G522 F524 K558 S561 R566 K616 M617 K618 K653 G733 N736 A739 Y1026 I1027 M1030 S1032 D1034 I1037 F1040	Y57 L164 Q167 I168 G270 F272 K306 S309 R314 K364 M365 K366 K401 G456 N459 A462 Y749 I750 M753 S755 D757 I760 F763
BS02	MG	A	A971 D1099	A694 D822

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003968 RNA-dependent RNA polymerase activity
• GO:0004519 endonuclease activity
• GO:0034062 5'-3' RNA polymerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0001172 RNA-templated transcription
• GO:0006351 DNA-templated transcription
• GO:0019079 viral genome replication
• GO:0039689 negative stranded viral RNA replication
• GO:0039694 viral RNA genome replication
• GO:0039696 RNA-templated viral transcription
• GO:0075526 cap snatching

Cellular Component

• GO:0030430 host cell cytoplasm
• GO:0044220 host cell perinuclear region of cytoplasm

External links

• PDB: RCSB:8qh3, PDBe:8qh3, PDBj:8qh3
• PDBsum: 8qh3
• PubMed: 38480734
• UniProt: P23456|L_HANTV RNA-directed RNA polymerase L (Gene Name=L)